merck millipore,默克密理博,03-179,RIPAb+™ SUZ12 - RIP Validated Antibody and Primer Set

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merck millipore,默克密理博,03-179,RIPAb+™ SUZ12 - RIP Validated Antibody and Primer Set
产品名称：RIPAb+™ SUZ12 - RIP Validated Antibody and Primer Set
产品型号：03-179
This RIPAb+ SUZ12 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,03-179,RIPAb+™ SUZ12 - RIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,03-179,RIPAb+™ SUZ12 - RIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
H	WB

描述
产品目录编号	03-179
品牌系列	Upstate
商名	Upstate RIPAb+
描述	RIPAb+™ SUZ12 - RIP Validated Antibody and Primer Set
概述	RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation. SUZ12 is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development.
Alternate Names	suppressor of zeste 12 homolog (Drosophila) ChET 9 protein Chromatin precipitated E2F target 9 protein Joined to JAZF1 protein Suppressor of zeste 12 protein homolog joined to JAZF1
背景信息	SUZ12 is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Component of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3.

产品信息
格式	Purified
控制	Includes negative control normal mouse IgG antibody and control primers specific for the cDNA of human lincRNA SFPQ.
演示	Anti-SUZ12 (Mouse Monoclonal) - One vial containing 50 μg of protein A purified antibody in PBS containing 0.1% azide, before the addition of 30% glycerol. Store at -20°C. Normal Mouse IgG - One vial containing 125 µg of purified mouse IgG in 125 µL of storage buffer containing 0.1% sodium azide. Store at -20°C. RIP Primers, lincSFPQ - One vial containing 75 μL of 5 μM of each primer specific for the human lincRNA SFPQ. Store at -20°C. FOR: TCG TAC TGT TAG GCC CTT GG REV: AAC CTT GCA TGA AGA GCA CC

应用
应用	This RIPAb+ SUZ12 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Western Blotting
应用说明	Immunoprecipitation from RIP lysate: Representative lot data. RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using either 5 µg of a normal mouse IgG (Cat. #CS200621), or 5 µg of Anti-SUZ12 antibody (Cat. # CS203201). 20 percent of the precipitated proteins (lane 1: mouse IgG, lane 2: SUZ12) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SUZ12 antibody (Cat. # CS203201, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00232) Arrow indicates SUZ12 (Figure 2). Western Blot Analysis: Representative lot data. Lysates from HeLa cells were resolved by electrophoresis, transferred to PVDF and probed with anti-SUZ12 (1:1,000 dilution). Proteins were visualized using goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection. Arrow SUZ12 (~95 kDa) (Figure 3).

生物信息
免疫原品种	Full length, recombinant human SUZ12.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	SUZ12
同种型	IgG1κ
品种反应性	Human
Species Reactivity Note	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_056170
Entrez基因汇总	This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. The specific function of this gene has not yet been determined.
基因符号	JJAZ1 SUZ12 KIAA0160 CHET9
纯化方法	Protein A purfied
UniProt编号	Q15022
UniProt汇总	FUNCTION: SwissProt: Q15022 # Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Component of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3. SIZE: 739 amino acids; 83055 Da SUBUNIT: Component of the PRC2 complex, composed of EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7. This complex is distinct from the PRC1 complex, which contains many other PcG proteins like BMI1, CBX4, CBX8, PHC2, PHC3, SCMH1, RING1 and RNF2. SUBCELLULAR LOCATION: Nucleus (Probable). DISEASE: SwissProt: Q15022 # A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer. SIMILARITY: SwissProt: Q15022 ## Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. & Contains 1 C2H2-type zinc finger.

产品使用声明
质量保证	RNA Binding Protein Immunoprecipitation: RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using either 5 µg of a normal mouse IgG , or 5 µg of Anti-SUZ12 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of SUZ12-associated RNA was verified by qPCR using RIP Primers lincSFPQ, (Figure 1) Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using either 5 µg of a normal mouse IgG , or 5 µg of Anti-SUZ12 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of SUZ12-associated RNA was verified by qPCR using RIP Primers lincSFPQ, (Figure 1)
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.