• merck millipore,默克密理博,ST1088,Anti-Akt, PH Domain Mouse mAb (SKB1)
  • merck millipore,默克密理博,ST1088,Anti-Akt, PH Domain Mouse mAb (SKB1)

    产品名称:Anti-Akt, PH Domain Mouse mAb (SKB1)
    产品型号:ST1088
    This Anti-Akt, PH Domain Mouse mAb (SKB1) is validated for use in Flow Cytometry, Immunoblotting, Immunoprecipitation for the detection of Akt, PH Domain.

    merck millipore,默克密理博,ST1088,Anti-Akt, PH Domain Mouse mAb (SKB1)

  • 产品介绍
  • merck millipore,默克密理博,ST1088,Anti-Akt, PH Domain Mouse mAb (SKB1)

    重要规格表

    宿主
    M
    描述
    概述 Recognizes the ~60 kDa Akt protein in A431 cells. Useful for the concentration of Akt in samples by immunoprecipitation prior to use in the K-LISA™ Akt Activity Kit (Cat. No. CBA019).
  • Antibody Target Gene Symbol: AKT1
  • Target Synonym: AKT, MGC99656, PKB, PKB-ALPHA, PKB/AKT, PRKBA, Protein kinase B, RAC, RAC-ALPHA, Thymoma viral proto-oncogene 1
  • Entrez Gene Name: v-akt murine thymoma viral oncogene homolog 1
  • Hu Entrez ID: 207 (Related Antibodies: 530311, PK1203, PC510 and 124001)
  • Mu Entrez ID: 11651
  • Rat Entrez ID: 24185
  • 产品目录编号 ST1088
    品牌系列 Calbiochem®
    同义词 Anti-PKB, Pleckstrin Homology Domain
    应用数据
    Detection of human Akt by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-Akt, PH Domain Mouse mAb (SKB1) (Cat. No. ST1088) (0.5 µg/ml). Detection: chemiluminescence.
    参考
    参考 Bellacosa, A., et al. 1998. Oncogene 17, 313.
    产品信息
    形式 Liquid
    配方 100 µg antibody in 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4 and 100 µg control lysate.
    正控制 A431 cells (included)
    防腐剂 ≤0.1% sodium azide
    应用
    应用参考 Immunoblotting Bourguignon, L.Y.W., et al. 2003. J. Biol. Chem. 278, 29420. Beffert, U., et al. 2002. J. Biol. Chem. 277, 49958. Immunoprecipitation, Active Enzyme Beffert, U., et al. 2002. J. Biol. Chem. 277, 49958.
    主要应用 Flow Cytometry
    Immunoblotting (Western Blotting)
    Immunoprecipitation
    生物信息
    免疫原 a recombinant protein consisting of amino acids 1-149 of human Akt 1 fused to GST
    免疫原品种 Human
    克隆 SKB1
    宿主 Mouse
    同种型 IgG
    品种 human, mouse, rat
    浓缩标签 Please refer to vial label for lot-specific concentration
    储存和货运信息
    货运代码 Shipped with Blue Ice or with Dry Ice
    毒性 Standard Handling
    储存 -20°C
    避免冻结 / 解冻 Avoid freeze/thaw
    无需冷冻 No
    特殊指南 Following initial thaw, aliquot and freeze (-20°C).

    merck millipore,默克密理博,ST1088,Anti-Akt, PH Domain Mouse mAb (SKB1)

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