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merck millipore,默克密理博,ST1013,PhosphoDetect™ Anti-GSK-3α/β (pTyr279/216) Rabbit pAb
产品名称：PhosphoDetect™ Anti-GSK-3α/β (pTyr279/216) Rabbit pAb
产品型号：ST1013
This PhosphoDetect™ Anti-GSK-3α/β Rabbit pAb is validated for use in Dot Blot, ELISA, WB, ICC for the detection of GSK-3α/β.
merck millipore,默克密理博,ST1013,PhosphoDetect™ Anti-GSK-3α/β (pTyr279/216) Rabbit pAb
- 产品介绍
重要规格表
宿主 Rb 描述 概述 Recognizes the ~51 kDa GSK-3α protein phosphorylated at Tyr279 and the ~47 kDa protein GSK-3β phosphorylated at Tyr216. Note: 1T = 1 test. Sufficient for 10 Western miniblots. 产品目录编号 ST1013 品牌系列 Calbiochem®
同义词 Anti-Glycogen Synthase Kinase-3α/β, Phospho-Specific (Tyr279/216) 应用数据 
Detection of human GSK-3β, phosphorylated at Tyr216 by immunoblotting. Samples: Whole cell lysate from control cells (lane 1) or control lysate spiked with human recombinant GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Glycogen Synthase Kinase-3α/β (pTyr279/216) Rabbit pAb (Cat. No. ST1013) (1:1000) pre-incubated without peptide (lanes 1, 5), or with phosphopeptide immunogen (lane 2), a generic phosphoserine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.参考 参考 Papasozomenos, S.C., et al. 2002. PNAS 99, 1140.
Kirschenbaum, F., et al. 2001. J. Biol. Chem. 276, 7366.
Bhat, R.V., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11074.产品信息 形式 Liquid 配方 In Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3. 负控制 Normal rabbit serum 正控制 Serum starved insulin-treated 3T3-L1 cells 防腐剂 ≤0.1% sodium azide 应用 主要应用 Dot Blot
Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
生物信息 免疫原 a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr279/216 phosphorylation sites of human GSK-3α/β 免疫原品种 Human 宿主 Rabbit 同种型 IgG 品种 human, mouse, rat 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-20°C).
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