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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AC113 描述 Anti-Neomycin Phosphotransferase II Antibody | AC113 Alternate Names - Neomycin-kanamycin phosphotransferase type II
- Aminoglycoside 3'-phosphotransferase
- APH(3')-II
- APH(3')II
- Kanamycin kinase, type II
背景信息 Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids. 产品信息 格式 Affinity Purified 控制 - Untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 主要应用 - Western Blotting
生物信息 免疫原品种 Recombinant protein corresponding to full length neomycin phosphotransferase (NPT) II. 宿主 Rabbit 特异性 This antibody reacts to lysates from cells transfected or transformed with a plasmid containing a kanamycin/neomycin resistance gene, nptII (Aminoglycoside 3'-phosphotransferase). 品种反应性 Human Species Reactivity Note Hek 293 +/- transfection with pUSEamp(-)and neomycin treatment. 抗体类型 Polyclonal Antibody Entrez基因编号 - YP_788126
基因符号 - nptII
- neo
- kan
纯化方法 Affinity purified UniProt编号 - P00552
产品使用声明 质量保证 Evaluated by Western Blot in untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Neomycin phosphotransferase II in 10 µg of neomycin treated HEK293 cells transfected with pUSEamp(-).使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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