merck millipore,默克密理博,SCR005,Accutase cell detachment solution

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merck millipore,默克密理博,SCR005,Accutase cell detachment solution
产品名称：Accutase cell detachment solution
产品型号：SCR005
A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cell More>>

merck millipore,默克密理博,SCR005,Accutase cell detachment solution

产品介绍

merck millipore,默克密理博,SCR005,Accutase cell detachment solution

描述
产品目录编号	SCR005
品牌系列	Chemicon®
商名	Chemicon
描述	Accutase cell detachment solution
概述	A cell detachment solution of proteolytic and collagenolytic enzymes. Useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including SmartPlastic™. ACCUTASE™ does not contain mammalian or bacterial derived products. Because the reagent does not contain universally proteolytic enzymes it may not release all cell/tissue types uniformly. Some optimization of cell type and passage number, as well as, incubation time and reagent strength typically needs to be performed to optimize the reagents effectiveness. ACCUTASE™ has been shown to be effective on a wide variety of cell types including: fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells. Each lot of ACCUTASE™ is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.

产品信息
演示	100 ml, ready to use, frozen sterile liquid. 1X ACCUTASE enzymes in Dulbecco's PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4) containing 0.5 mM EDTA·4Na and 3 mg/L Phenol Red.

应用
应用	A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.
应用说明	Cell Detachment 1. Thaw ACCUTASE™ at room temperature. 2. Wash plate, flask or beads with sterile PBS. 3. Add ACCUTASE™ to culture dish or flask using aseptic procedures at 10 ml per 75-cm2 surface area. 4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes). 5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required Optimal incubation times and reagent strength needs to be determined for each cell/tissue type tested by the enduser.

应用

A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

应用说明

Cell Detachment

1. Thaw ACCUTASE™ at room temperature.

2. Wash plate, flask or beads with sterile PBS.

3. Add ACCUTASE™ to culture dish or flask using aseptic procedures at 10 ml per 75-cm2 surface area.

4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).

5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required

Optimal incubation times and reagent strength needs to be determined for each cell/tissue type tested by the enduser.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable when stored at -20°C. Refer to lot expiration date. Recommended storage upon receipt is -20°C. After thawing, ACCUTASE may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMP