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merck millipore,默克密理博,SCC066,Blood-Brain Barrier hCMEC/D3 Cell Line
产品名称：Blood-Brain Barrier hCMEC/D3 Cell Line
产品型号：SCC066
The hCMEC/D3 BBB cell line was derived from human temporal lobe microvessels and were immortalized with hTERT and SV40 large T antigen. The cells have been extensively characterized for brain endothel More>>
merck millipore,默克密理博,SCC066,Blood-Brain Barrier hCMEC/D3 Cell Line
- 产品介绍
重要规格表
主要应用 CULT 描述 产品目录编号 SCC066 描述 Blood-Brain Barrier hCMEC/D3 Cell Line Alternate Names - hCMEC/D3 cell line
- Human BBB cell line
- Human blood-brain barrier cell line
- Human cerebral endothelial cell line
- CMEC/D3 cell Line
背景信息 This product is available for sale to academic institutions or not-for-profit entities for research use.
The blood–brain barrier (BBB) is a highly selective permeability barrier that separates the circulating blood from the brain extracellular fluid in the central nervous system. The blood–brain barrier is formed by capillary endothelial cells, which are connected by tight junctions with an extremely high electrical resistivity. The blood–brain barrier allows the passage of water, some gases, and lipid soluble molecules by passive diffusion, as well as the selective transport of molecules such as glucose and amino acids that are crucial to neural function. On the other hand, the blood–brain barrier may prevent the entry of lipophilic, potential neurotoxins by way of an active transport mechanism mediated by P-glycoprotein. Astrocytes are necessary to create the blood–brain barrier. The blood–brain barrier (BBB) prevents entry into the brain of most drugs from the blood. The presence of the BBB makes difficult the development of new treatments of brain diseases, or new radiopharmaceuticals for neuroimaging of brain.
The hCMEC/D3 cell line was derived from human temporal lobe microvessels isolated from tissue excised during surgery for control of epilepsy. The primary isolate was enriched in cerebral endothelial cells (CECs). In the first passage, cells were sequentially immortalized by lentiviral vector transduction with the catalytic subunit of human telomerase (hTERT) and SV40 large T antigen, following which CEC were selectively isolated by limited dilution cloning, and clones were extensively characterized for brain endothelial phenotype. This brain microvascular endothelial cell line represents one such model of the human BBB that can be easily grown and is amenable to cellular and molecular studies on pathological and drug transport mechanisms with relevance to the central nervous system (CNS).
Reference:
Couraud PO, et al. (2008) The human brain endothelial cell line hCMEC/D3 as a human blood‐brain barrier model for drug transport studies. Fluids Barriers CNS. 2013 Mar 26;10(1):16.产品信息 部件 - 1) ≥1X106 viable Blood-Brain Barrier hCMEC/D3 Cells: (Catalog No. SCC066). Store in liquid nitrogen.
应用 应用 The hCMEC/D3 BBB cell line was derived from human temporal lobe microvessels and were immortalized with hTERT and SV40 large T antigen. The cells have been extensively characterized for brain endothelial phenotype and are a model of human blood-brain barrier (BBB) function. 主要应用 - Cell Culture
应用说明 This product is available for sale to academic institutions or not-for-profit entities for research use. 生物信息 品种 Human 产品使用声明 质量保证 • Each vial contains ≥ 1X10^6 viable cells.
• Cells are tested by PCR and are negative for Hepatitis A, B, C, HPV, Herpes and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Blood-Brain Barrier hCMEC/D3 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality. 包装信息 数量 ≥1X10⁶ cells/vial
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