- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,S7150,OxyBlot Protein Oxidation Detection Kit
产品名称：OxyBlot Protein Oxidation Detection Kit
产品型号：S7150
The OxyBlot Protein Oxidation Detection Kit provides the reagents to perform the immunoblot detection of carbonyl groups introduced into proteins by oxidative reactions with ozone or oxides of nitroge More>>
merck millipore,默克密理博,S7150,OxyBlot Protein Oxidation Detection Kit
- 产品介绍
重要规格表
主要应用 WB 描述 产品目录编号 S7150 品牌系列 Chemicon®
商名 - Chemicon
- OxyBlot
描述 OxyBlot Protein Oxidation Detection Kit 概述 The OxyBlot™ Protein Oxidation Detection Kit provides the chemical and immunological reagents necessary to perform the immunoblot detection of carbonyl groups introduced into proteins by oxidative reactions with ozone or oxides of nitrogen or by metal catalyzed oxidation. 产品信息 检测方法 Chemiluminescence 应用 应用 The OxyBlot Protein Oxidation Detection Kit provides the reagents to perform the immunoblot detection of carbonyl groups introduced into proteins by oxidative reactions with ozone or oxides of nitrogen or by metal catalyzed oxidation. 主要应用 - Western Blotting
应用说明 Preparation of Cell Lysate
1. Wash adherent cells twice in the dish or flask with ice-cold PBS and drain off PBS. Wash non-adherent cells in PBS and centrifuge at 800 to 1000 rpm in a table-top centrifuge for 5 minutes to pellet the cells.
2. Add ice-cold RIPA buffer to cells (1 ml per 107 cells/100 mm dish/150 cm2 flask; 0.5 ml per 5 x 106 cells/60 mm dish/75 cm2 flask).
3. Scrape adherent cells off the dish or flask with either a rubber policeman or a plastic cell scraper that has been cooled in ice-cold distilled water. Transfer the cell suspension into a centrifuge tube. Gently rock the suspension on either a rocker or an orbital shaker in the cold room for 15 minutes to lyse cells.
4. Centrifuge the lysate at 14,000 x g in a precooled centrifuge for 15 minutes. Immediately transfer the supernatant to a fresh centrifuge tube and discard the pellet.
5. Dilute the cell lysate at least 1 : 10 before determining the protein concentration because of the interference of the detergents in the lysis buffer with the Coomassie-based reagent. At this step, the sample can be divided into aliquots and stored at -20¡C for up to a month.
TIP: When working with large volumes of non-adherent cells, the cells may not be cooled quickly enough to maintain the activity of the protein being studied. In this case, pour the cell suspension into a mixture of an equal mass of 2 x PBS and ice, then collect the cells by centrifugation and perform the lysis as described above.
RIPA lysis buffer:
Catalog number 20-188
Reducing agent is very important for preventing oxidation:
The protein lysate may be prepared using a variety of protocols. Most standard buffers such as RIPA and Triton are also suitable. It is recommended that a reducing agent be added to the lysis buffer to prevent the oxidation of proteins that may occur after cell lysis. Lysis buffer containing either 1-2% 2-mercaptoethanol or 50 mM DTT sufficiently inhibits this oxidation, but has no adverse effect on the derivatization reaction in the OxyBlot™ protocol. Purified protein samples are also suitable for analysis with the OxyBlot™ Kit.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store all components at 2°C to 8°C. Store the protein standard (component 90450) at -25° to -15°C within 2 weeks of arrival. 包装信息 数量 200 assays
上一件merck millipore产品：merck millipore,默克密理博,309795,DIDS, Disodium Salt - CAS 53005-05-3 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,521235,PDGFR Tyrosine Kinase Inhibitor VI, SU6668 - CAS 210644-62-5 - Calbiochem
相关产品
merck millipore,默克密理博,GF146,Wnt-5a Protein, Recombinant mouse
merck millipore,默克密理博,AB5208-50UL,Anti-Sodium Channel Antibody, Voltage Gated, Brain Type III, pain
merck millipore,默克密理博,208783,Calmodulin, Porcine Brain
merck millipore,默克密理博,309795,DIDS, Disodium Salt - CAS 53005-05-3 - Calbiochem
merck millipore,默克密理博,SCM055,AOF ITS Supplement (100X), 100ml,SCM055
merck millipore,默克密理博,MAB2638,Anti-Integrin beta7 Antibody, clone TN114
merck millipore,默克密理博,20-177,100X GDP, 100mM
merck millipore,默克密理博,05-434,Anti-Syk Antibody, clone 4D10.1