merck millipore,默克密理博,04-1081,Anti-Calcium/Calmodulin Kinase IV Antibody, clone EP2564Y, rabbit monoclonal

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merck millipore,默克密理博,04-1081,Anti-Calcium/Calmodulin Kinase IV Antibody, clone EP2564Y, rabbit monoclonal
产品名称：Anti-Calcium/Calmodulin Kinase IV Antibody, clone EP2564Y, rabbit monoclonal
产品型号：04-1081
Anti-Calcium/Calmodulin Kinase IV Antibody, clone EP2564Y, Rabbit is an antibody against Calcium/Calmodulin Kinase IV for use in WB & IC.

merck millipore,默克密理博,04-1081,Anti-Calcium/Calmodulin Kinase IV Antibody, clone EP2564Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1081,Anti-Calcium/Calmodulin Kinase IV Antibody, clone EP2564Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1081
描述	Anti-Calcium/Calmodulin Kinase IV Antibody, clone EP2564Y, rabbit monoclonal
Alternate Names	calcium/calmodulin-dependent protein kinase type IV<br />catalytic chain CAM kinase IV CAM kinase- GR CAM kinase-GR CaMK IV brain Ca(2+)-calmodulin-dependent protein kinase type IV brain Ca++-calmodulin-dependent protein kinase type IV calcium/calmodulin-dependent protein kinase IV
背景信息	The Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.

产品信息
格式	Unpurified
控制	Jurkat and Human brain lysates
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Anti-Calcium/Calmodulin Kinase IV Antibody, clone EP2564Y, Rabbit is an antibody against Calcium/Calmodulin Kinase IV for use in WB & IC.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.

生物信息
免疫原品种	Synthetic peptide corresponding to residues near the N-terminus of human Calcium/Calmodulin Kinase IV.
表位	N-terminus
克隆	EP2564Y
宿主	Rabbit
特异性	This antibody recognizes Calcium/Calmodulin Kinase IV.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse, and rat
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_001744.3
Entrez基因汇总	The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells
基因符号	MGC36771 CaMK-GR EC 2.7.11.17
纯化方法	Unpurified
UniProt编号	Q16566
UniProt汇总	FUNCTION: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory By similarity. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. ENZYME REGULATION:Activated by Ca2+/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2. Autophosphorylation of the N-terminus is required for full activation. In part, activity is independent on Ca2+/calmodulin and autophosphorylation of Ser-336 allows to switch to a Ca2+/calmodulin-independent state By similarity. Probably inactivated by serine/threonine protein phosphatase 2A. SUBUNIT STRUCTURE: Monomer By similarity. Interacts with serine/threonine protein phosphatase 2A catalytic subunit, PPP2CA or PPP2CB. The interaction with PP2CA or PP2CB is mutually exclusive with binding to Ca2+/calmodulin. SUBCELLULAR LOCATION: Cytoplasm By similarity. Nucleus By similarity. Note: Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix By similarity. TISSUE SPECIFICTY: Expressed in epithelial ovarian cancer tissue. Ref.8 PTM: Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 By similarity. Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.Contains 1 protein kinase domain.

产品使用声明
质量保证	Evaluated by Western Blot on Jurkat and Human brain lysates. Western Blot Analysis: A 1:1,000-2,000 dilution of this antibody was used to detect Calcium/Calmodulin Kinase IV in Jurkat and Human brain lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.