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merck millipore,默克密理博,RCI1MAG-87K,MILLIPLEX MAP Rat Cardiac Injury Panel 1 - Cardiotoxicity Multiplex Assay
产品名称：MILLIPLEX MAP Rat Cardiac Injury Panel 1 - Cardiotoxicity Multiplex Assay
产品型号：RCI1MAG-87K
The MILLIPLEX MAP Rat Cardiac Injury Magnetic Bead Panel 1 is a configurable 7-plex kit for the quantification of cTnI, cTnT, CKM (MM-CK), FABP3, FSTL1, MYL3, and TIMP-1 in serum or pl More>>
merck millipore,默克密理博,RCI1MAG-87K,MILLIPLEX MAP Rat Cardiac Injury Panel 1 - Cardiotoxicity Multiplex Assay
- 产品介绍
- merck millipore,默克密理博,RCI1MAG-87K,MILLIPLEX MAP Rat Cardiac Injury Panel 1 - Cardiotoxicity Multiplex Assay
重要规格表
可用的分析物 品种反应性 主要应用 检测方法 TIMP-1, Troponin I (TnI), Troponin T (TnT), Creatine Kinase, Muscle (CKM), FABP3, Follistatin-like Protein 1 (FSTL1), Myosin Light Chain 3 (MYL3) R Mplex Luminex xMAP 描述 产品目录编号 RCI1MAG-87K 商名 - MILLIPLEX
描述 MILLIPLEX MAP Rat Cardiac Injury Panel 1 - Cardiotoxicity Multiplex Assay 概述 The MILLIPLEX® MAP Rat Cardiac Injury Magnetic Bead Panel 1 (RCI1MAG-87K) contains all the components necessary to simultaneous quantify any or all of the following 7 analytes in serum and plasma samples:
• Cardiac Troponin I (cTnI)
• Cardiac Troponin T (cTnT)
• Creatine Kinase, Muscle (CKM)
• Fatty Acid Binding Protein 3 (FABP3)
• Follistatin-like Protein 1 (FSTL1)
• Myosin Light Chain 3 (MYL3)
• Tissue Inhibitor of Metalloproteinase-1 (TIMP-1)
This kit uses a 96-well format, contains lyophilized standard cocktail and two quality controls and can measure up to 38 serum or plasma samples in duplicate.背景信息 Drug-induced cardiotoxicity can be life threatening and necessitates limiting an otherwise-effective treatment. In fact, cardiovascular toxicity has been reported to account for over 40% of drug candidate withdrawal in premarketing clinical trials.
Many therapeutic agents have been reported to induce or exacerbate heart failure including anti-tumor medications, immunomodulating drugs, antidepressants and calcium channel blocking agents to name a few. Conventional biomarkers of cardiotoxicity such as aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine kinase (CK) have gradually been withdrawn due to low tissue specificity. The number of cardiac-specific biomarkers adapted from clinical studies of cardiotoxicity continues to rise. The reverse translational application of these auspicious biomarkers in experimental animal models could improve the power of detection for diagnosis, helping to predict the occurrence of cardiotoxicity in humans.
Emerging biomarkers of cardiac injury include multiple categories of proteins: characteristic muscle cell proteins (cTnI, cTnT, FABP3 and MYL3), cytosolic kinase (CKM), secreted heparin-binding protein (FSTL1) and metalloprotease inhibitor (TIMP1). Numerous studies have demonstrated elevated levels of these biomarkers in experimental animal blood samples. In order to meet the increasing need to provide screening assays for experimental biomarkers of drug-induced cardiac injury in pre-clinical and translational research models, EMD Millipore has developed the MILLIPLEX® MAP Rat Cardiac Injury Magnetic Bead Panel 1. This confugurable 7-plex panel is the most versatile system available for cardiac injury screening in pre-clinical and translational research models.产品信息 部件 - 2 Foil Plate Sealers
- Rat Cardiac Injury Standard, 1 vial, lyophilized
- Rat Cardiac Injury Quality Control 1, 1 vial, lyophilized
- Rat Cardiac Injury Quality Control 2, 1 vial, lyophilized
- Serum Matrix (0.08% Sodium Azide), 1 vial, lyophylized
- Assay Buffer, 1 bottle, 30 mL
- Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
- Rat Cardiac Injury Panel 1 Detection Antibodies, 1 bottle, 3.2 mL
- Rat Cardiac Injury Panel 1 Antibody-Immobilized Beads (20X), 200 µL ea
- Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL
- Mixing Bottle, 1 bottle
- Sample Plate, 96 well format, 1 plate
检测方法 Luminex xMAP 配置 Design your multiplex kit by choosing analytes. 精度（%） - Intra-assay: <10
- Inter-assay: <15
板面类型 MAGNETIC Toxicity 应用 应用 The MILLIPLEX® MAP Rat Cardiac Injury Magnetic Bead Panel 1 is a configurable 7-plex kit for the quantification of cTnI, cTnT, CKM (MM-CK), FABP3, FSTL1, MYL3, and TIMP-1 in serum or plasma samples on the Luminex xMAP® instrument platform. 主要应用 - Multiplexing
应用说明 • This assay requires 25 µL of 1:5 diluted serum or plasma sample per well in kit Assay Buffer (please dilute per protocol instructions).
• This assay protocol includes instruction for 2 Hour or Overnight incubation.生物信息 特异性 There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. 品种反应性 Rat 可用的分析物 - TIMP-1
- Troponin I (TnI)
- Troponin T (TnT)
- Creatine Kinase, Muscle (CKM)
- FABP3
- Follistatin-like Protein 1 (FSTL1)
- Myosin Light Chain 3 (MYL3)
化验面板 Everything you need in a single kit. 交叉反应性 There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. 准确度 - Please refer to Assay Protocol for individual analyte accuracy.
- 2 Hour Protocol: 80% - 100% Recovery
- Overnight Protocol: 86% - 103% Recovery
理化信息 灵敏度 - Please see kit protocol for assay sensitivities of individual analytes.
- 2 Hour Protocol: 9 - 86 pg/mL (MinDC+2SD).
- Overnight Protocol: 7 - 155 pg/mL (MinDC+2SD).
稀释的线性 Contact technical support for this information. 标准曲线范围 - TIMP-1: 41 – 30,000 pg/mL
- cTnI: 123 – 90,000 pg/mL
- cTnT: 82 – 60,000 pg/mL
- CKM: 21 – 15,000 pg/mL
- FABP3: 41 – 30,000 pg/mL
- FSTL1: 82 – 60,000 pg/mL
- MYL3: 4 – 3,000 pg/mL
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Recommended storage for kit components is 2 - 8°C. 包装信息 数量 96-well plate
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