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  • merck millipore,默克密理博,RADPCMAG-82K,MILLIPLEX MAP Rat Adipocyte Panel - Metabolism Assay

  • merck millipore,默克密理博,RADPCMAG-82K,MILLIPLEX MAP Rat Adipocyte Panel - Metabolism Assay

    产品名称：MILLIPLEX MAP Rat Adipocyte Panel - Metabolism Assay
    产品型号：RADPCMAG-82K
    The MILLIPLEX MAP Rat Adipocyte Magnetic Bead Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysates and cu More>>

    merck millipore,默克密理博,RADPCMAG-82K,MILLIPLEX MAP Rat Adipocyte Panel - Metabolism Assay

  • 产品介绍
  • merck millipore,默克密理博,RADPCMAG-82K,MILLIPLEX MAP Rat Adipocyte Panel - Metabolism Assay

    重要规格表

    可用的分析物 品种反应性 主要应用 检测方法
    TNFα, Adiponectin, IL-1β, IL-6, Leptin, MCP-1, PAI-1 (Total)RMplexLuminex xMAP
    描述
    产品目录编号 RADPCMAG-82K
    商名
    • MILLIPLEX
    描述 MILLIPLEX® MAP Rat Adipocyte Panel - Metabolism Assay
    概述 The Milliplex® MAP Rat Adipocyte Magnetic Bead Panel contains all the components necessary to measure 7 biomarkers in any combination: Adiponectin, IL-1β, IL-6, Leptin, MCP-1, PAI-1 Total, and TNFα. The kit uses a 96-well format, contains a lyophilized standard cocktail, 2 quality controls and can measure up to 38 serum or plasma samples in duplicate.
    背景信息 In the past, the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete a number of hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. One of these Adipokines, Adiponectin, is involved in the regulation of lipid and glucose metabolism, influencing the body’s response to insulin. Also, its anti-inflammatory effects on the cellular lining of blood vessel walls may help explain the association of high Adiponectin levels with the reduced risk of heart attack.

    The MILLIPLEX® MAP Rat Adipocyte Magnetic Bead Panel contains carefully selected biomarkers, enabling researchers to focus on the therapeutic potential of adipokines, adipocytes and cytokine biomarkers simultaneously. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative detection of multiple analytes simultaneously, which can dramatically improve productivity.
    产品信息
    部件
    • 2 Foil Plate Sealers
    • Rat Adipokine Standard, 1 vial, lyophilized
    • Rat Adipokine Quality Control 1, 1 vial, lyophilized
    • Rat Adipokine Quality Control 2, 1 vial, lyophilized
    • Assay Buffer, 1 bottle, 30 mL
    • Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
    • Rat Adipocyte Detection Antibodies, 1 bottle, 5.5 mL
    • Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
    • Mixing Bottle, 1 bottle
    • Sample Plate, 96 well format, 1 plate
    检测方法 Luminex xMAP
    配置 Design your multiplex kit by choosing analytes.
    精度（%）
    • Intra-Assay: < 10
    • Inter-assay: < 15
    板面类型 MAGNETIC Metabolism
    应用
    应用 The MILLIPLEX® MAP Rat Adipocyte Magnetic Bead Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysates and culture supernatant samples: Adiponectin, IL-1β, IL-6, Leptin, MCP-1, PAI-1 Total, and TNFα.
    主要应用
    • Multiplexing
    应用说明 This assay is an overnight assay. Each well requires 25 μL of sample, standard or control. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay. Tissue/cell extracts should be done in neutral buffers containing reagents and conditions that do not interfere with assay performance. The tissue/cell extract samples should be free of particles such as cells or tissue debris.
    生物信息
    特异性 There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
    品种反应性 Rat
    可用的分析物
    • TNFα
    • Adiponectin
    • IL-1β
    • IL-6
    • Leptin
    • MCP-1
    • PAI-1 (Total)
    化验面板 Everything you need in a single kit.
    交叉反应性 There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
    准确度
    • 94% - 121%
    理化信息
    灵敏度
    • Please see kit protocol for individual assay sensitivities.
    • 0.1 - 25 pg/mL (MinDC+2SD).
    稀释的线性 Contact technical support for this information.
    标准曲线范围
    • Adiponectin: 122 – 500,000 pg/mL
    • IL-1β, TNFα: 0.7 – 3,000 pg/mL
    • MCP-1: 9.8 – 40,000 pg/mL
    • PAI-1: 12 – 50,000 pg/mL
    • IL-6, Leptin: 15 – 60,000 pg/mL
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Recommended storage for kit components is 2 - 8°C.
    包装信息
    数量 96-well plate

    merck millipore,默克密理博,RADPCMAG-82K,MILLIPLEX MAP Rat Adipocyte Panel - Metabolism Assay

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