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品种反应性 主要应用 宿主 格式 抗体类型 M, H ICC, WB Rb Purified Polyclonal Antibody 描述 产品目录编号 09-403 描述 Anti-TKS5 (SH3 #1) Antibody Alternate Names - five SH3 domains
- Adaptor protein TKS5
- Five SH3 domain-containing protein
- SH3 and PX domains 2A
- SH3 multiple domains 1
- SH3 multiple domains protein 1
背景信息 Tks5 (previously known as Fish), is a large scaffolding protein with one amino-terminal PX domain and five SH3 domains. It is cytoplasmic in normal fibroblasts. In Src-transformed cells, Tks5 localizes to podosomes. The coexpression of Tks5 and constitutively active Src in epithelial cancer cells resulted in the appearance of podosomes/invadopodia. Cells with reduced Tks5 expression are poorly invasive through matrigel. Tks5 is expressed and localized to podosomes/invadopodia in some invasive human cancer cell lines and tumor tissue, particularly breast cancers and melanomas. Thus, Tks5 appears to be required for podosome/invadopodia formation and for invasion of some cancer cells. 产品信息 格式 Purified 控制 - Western Blot::
NIH-3T3 (100% confluent) lysate
Immunocytochemistry:
Mouse 3T3-Src(Y527F) cells
演示 Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3. 应用 应用 Anti-TKS5 (SH3 #1) Antibody detects level of TKS5 (SH3 #1) & has been published & validated for use in WB, IC & IP. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western Blot Analysis: Antibody detected TKS5 in 20 μg of NIH-3T3 (100% confluent) lysate.
生物信息 免疫原品种 Recombinant Protein, aa: 166-225. Homology: Human, Dog, Horse, Rat, Cow (100%, 60/60); Chicken (93%, 56/60) 表位 166-225 aa 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 MouseHuman Species Reactivity Note Reacts with Mouse and Human. Other homologies: Human, Canine, Equine, Rat, and Bovine (100% sequence homology), Chicken (93% sequence homology) 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_008018.3
基因符号 - MGC144076
- FISH
- KIAA0418
- SH3MD1
- Sh3pxd2a
纯化方法 Protein A purfied UniProt编号 - O89032
UniProt汇总 Function: Required for podosome formation, degradation of the extracellular matrix, and for the invasiveness of some cancer cells. Binds phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,4-biphosphate (PtdIns(3,4)P2). In association with ADAM12, mediates the neurotoxic effect of beta-amyloid peptide By similarity.
Subunit: structure Interacts with ADAM12, ADAM15 and ADAM19 By similarity.
Subcellular location: Cytoplasm. Cell projection › podosomeBy similarity. Note= Cytoplasmic in normal cells and localizes to podosomes in Src-transformed cells By similarity.
Tissue specificity: Widely expressed. Not found in the spleen and testis. Ref.1
Domain: The PX domain is required for podosome localization, and for binding phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,4-biphosphate (PtdIns(3,4)P2) By similarity.
The fifth SH3 domain mediates binding with ADAM12, ADAM15 and ADAM19 By similarity.
Post-translational modification: Tyrosine phosphorylated by SRC. Phosphorylation plays a regulatory role in the protein localization. The intramolecular interaction of the PX domain with the third SH3 domain maintains the protein in the cytoplasm and phosphorylation disrupts this interaction, resulting in the redistribution of the protein from cytoplasm to the perimembrane region. Phosphorylated on serine upon DNA damage, probably by ATM or ATR By similarity.
Sequence similarities: Belongs to the SH3PXD2 family.
Contains 1 PX (phox homology) domain.
Contains 5 SH3 domains.
产品使用声明 质量保证 Routinely evaluated by Western Blot on NIH-3T3 (100% confluent) lysate. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. 包装信息 数量 100 µg
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