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描述 概述 This kit provides a unique combination of reagents useful for the isolation of a highly enriched mitochondrial fraction from the cytosol. The enriched fractions can be used to study factors of interest using Western blotting, ELISA, or other assays. Note: 1 Test = reagents sufficient for processing 5 x 107 cells. 产品目录编号 QIA88 品牌系列 Calbiochem®
应用数据 
Mitochondrial and cytosolic fractions were isolated from HEPG2 cells using QIA88 ProteoExtract® Cytosol/ Mitochondria Fractionation Kit. Mitochondrial pellets were resuspended in PBS. The protein concentration of each fraction was determined using the BCA assay. DTT was omitted from the Cytosol Extraction Buffer as it can interfere with concentration determination in the BCA assay. Equivalent amounts (10 µg) of HEPG2 homogenate (total protein), cytosolic, and mitochondrial fractions were analyzed by Western blot for proteins localized to different cellular regions as indicated. Representative results from 2 independent fractionations are shown.产品信息 形式 100 Extractions 格式 Cell extraction 试剂盒包含 Mitochondria Extraction Buffer, Cytosol Extraction Buffer, Protease Inhibitor Cocktail, Reducing Reagent, and a user protocol. 生物信息 样品类型 Cells 安全信息 R相 R: 23/24/25-32-34-36/37/38-41
Toxic by inhalation, in contact with skin and if swallowed.
Contact with acids liberates very toxic gas.
Causes burns.
Irritating to eyes, respiratory system and skin.
Risk of serious damage to eyes.S相 S: 23-26-36/37/39-45
Do not breathe fumes.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).产品使用声明 预定用途 The ProteoExtract® Cytosol/Mitochondria Fractionation Kit is designed for extraction of mammalian cells to yield enriched mitochondrial and cytosolic fractions for use in downstream analyses, such as Western blotting. 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
有害材料注意事项： Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存 -20°C
存储条件 Upon arrival store the entire contents of the kit at -20°C. Following initial use, store the buffers at 4°C and the DTT and Protease Inhibitor Cocktail at -20°C. 避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 Mitochondria Extraction Buffer, Cytosol Extraction Buffer, Protease Inhibitor Cocktail, Reducing Reagent, and a user protocol.
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