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merck millipore,默克密理博,48-690MAG,MILLIPLEX MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex - Cell Signaling Multiplex Assay
产品名称:MILLIPLEX MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex - Cell Signaling Multiplex Assay
产品型号:48-690MAG
The analytes available for this multiplex kit are: CD3ε (pan Tyr), CREB (S133), ERK (Thr185/Tyr187), LAT (pan Tyr), Lck (pan Tyr), Syk (pan Tyr), ZAP-70 (pan Tyr).
merck millipore,默克密理博,48-690MAG,MILLIPLEX MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex - Cell Signaling Multiplex Assay
- 产品介绍
- merck millipore,默克密理博,48-690MAG,MILLIPLEX MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex - Cell Signaling Multiplex Assay
重要规格表
可用的分析物 品种反应性 主要应用 检测方法 ZAP-70 (pan Tyr), CD3ε (pan Tyr), CREB (S133), ERK (Thr185/Tyr187), LAT (pan Tyr), Lck (pan Tyr), Syk (pan Tyr) H Mplex Luminex xMAP 描述 产品目录编号 48-690MAG 商名 - MILLIPLEX
描述 MILLIPLEX MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex - Cell Signaling Multiplex Assay 概述 The MILLIPLEX® MAP 7-Plex Human T-Cell Receptor (TCR) Magnetic Bead Kit is used to detect changes in phosphorylated CREB (Ser133), Erk/MAP kinase 1/2 (Thr185/Tyr187), and phosphorylated tyrosine residues on CD3 epsilon chain, Lck, ZAP-70, LAT, and Syk in T-cell lysates using the Luminex® system. Although the protein kinase Syk is not part of the T-Cell Receptor pathway, it does play a significant role in B-Cell Receptor signaling and acts as a negative control for the analysis of T-Cell Receptor signaling. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. 产品信息 检测方法 Luminex xMAP 配置 Premixed 精度(%) - Inter-Assay: 4% - 17%
- Intra-Assay: 3% - 8%
板面类型 Cell Signaling 应用 应用 The analytes available for this multiplex kit are: CD3ε (pan Tyr), CREB (S133), ERK (Thr185/Tyr187), LAT (pan Tyr), Lck (pan Tyr), Syk (pan Tyr), ZAP-70 (pan Tyr). 主要应用 - Multiplexing
应用说明 • An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).生物信息 特异性 Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. 品种反应性 Human 可用的分析物 - ZAP-70 (pan Tyr)
- CD3ε (pan Tyr)
- CREB (S133)
- ERK (Thr185/Tyr187)
- LAT (pan Tyr)
- Lck (pan Tyr)
- Syk (pan Tyr)
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Recommended storage for kit components is 2 - 8°C 包装信息 数量 96-well plate
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