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检测方法 Fluorometric 描述 概述 Suitable for the in vitro detection of caspase-3 activity in cell lysates. The assay is simple, rapid, and includes all controls. Suitable for use in frozen samples. Assay is validated against the Nucleosome ELISA Kit (Cat. No. QIA25) and the Cell Death Detection NMP ELISA Kit (Cat. No. QIA20). A high-throughput drug-screening version is also available (Cat. No. HTS02). 产品目录编号 QIA70 品牌系列 Calbiochem®
参考 参考 X. Sun, et al. 1999. J. Biol. Chem. 274, 5053.
N.A. Thornberry and Y. Lazebnik, 1998. Science 281, 1312.
S. J. Martin and D. 1995 R. Green, Cell 82, 349.产品信息 检测方法 Fluorometric 形式 96 Tests 格式 96-well plate 试剂盒包含 96-Well Plate, Caspase-3 Extraction Buffer, Assay Buffer, Recombinant Caspase-3, Caspase-3 Substrate Conjugate, Caspase-3 Inhibitor, Control Cell Pellets, and a user protocol. 生物信息 化验时间 2.5-3 h 样品类型 Cell lysates 品种 a broad range of species 安全信息 R相 R: 36/37/38
Irritating to eyes, respiratory system and skin.S相 S: 26-36-23
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Do not breathe fumes.产品使用声明 预定用途 The Calbiochem Caspase-3 Activity Assay is a non-isotopic enzyme assay for the in vitro detection of Caspase-3 activity in cell lysates. The assay can be adapted for 96-well plates for sensitive drug screening methods. 储存和货运信息 货运代码 Multiple Storage Conditions
毒性 Multiple Toxicity Values, refer to MSDS
储存 Multiple storage requirements
存储条件 Upon Receipt of the kit: Store Recombinant Caspase-3 (2 vials) at -80°C to maintain stability. Store all other kit components at -20°C. Once thawed, Caspase-3 Sample Buffer and Assay Buffer may be stored at 4°C. Do not expose reagents to excessive light. 防潮 Protect from moisture
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 96-Well Plate, Caspase-3 Extraction Buffer, Assay Buffer, Recombinant Caspase-3, Caspase-3 Substrate Conjugate, Caspase-3 Inhibitor, Control Cell Pellets, and a user protocol.
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