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描述 产品目录编号 14-347M 品牌系列 Upstate
商名 - Upstate
描述 CHK2 Protein, active, 250 µg 概述 Recombinant human CHK2, residues 5-543, containing N-terminal GST and Cterminal His6 tags 应用 应用 Active, recombinant human CHK2, residues 5-543, containing N-terminal GST & C-terminal His-6 tags, for use in Kinase Assays. 主要应用 - Kinase Assay
生物信息 来源 Expressed in E. coli 品种 Human 特异活性 For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. Entrez基因编号 - NM_145862.2
- NM_001005735.1
- NM_007194.3
Entrez基因汇总 In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. 基因符号 - Cds1
- CHEK2
- HuCds1
- CHK2
- LFS2
- RAD53
- bA444G7
- CDS1
- OTTHUMP00000028871
- PP1425
蛋白靶 CHK2 纯化方法 glutathione-agarose and Ni2+/NTA-agarose 靶子系列 CAMK UniProt编号 - O96017
UniProt汇总 FUNCTION: SwissProt: O96017 # Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
COFACTOR: Magnesium.
SIZE: 543 amino acids; 60915 Da
SUBCELLULAR LOCATION: Isoform 2: Nucleus. Note=Isoform 10 is present throughout the cell. & Isoform 4: Nucleus. & Isoform 7: Nucleus. & Isoform 9: Nucleus. & Isoform 12: Nucleus.
TISSUE SPECIFICITY: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
DISEASE: SwissProt: O96017 # Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. & Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807]. & Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500].
SIMILARITY: SwissProt: O96017 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily. & Contains 1 FHA domain. & Contains 1 protein kinase domain.产品使用声明 质量保证 routinely evaluated by phosphorylation of the CHKtide substrate peptide 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 1 year at -70°C 包装信息 数量 250 µg 包装 Also available in 10 μg size (cat#14-347) and in bulk (cat# 14-347-K).
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