merck millipore,默克密理博,PMEF-NX,EmbryoMax Primary Mouse Embryo Fibroblasts, Neo Resistant, Irradiated, passage 3

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Merck Millipore 默克密理愽

主要应用
Cult

描述
产品目录编号	PMEF-NX
商名	Specialty Media Embryomax
描述	EmbryoMax® Primary Mouse Embryo Fibroblasts, Neo Resistant, Irradiated, passage 3
概述	These neomycin resistant PMEF cells were isolated from FVB mouse embryos at day 13 and mitotically inactivated by treatment with irradiation. The cells can be directly plated onto gelatin coated (Cat. No. ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 10⁶ cells will produce the appropriate density feeder layer on the following tissue culture vessels: • 6 x 60mm dishes • 2 x T75 flasks • 2 x 6 well plates Refer to Murine Embryonic Stem Cell Culture Manual for complete culture protocols and media formulations for PMEF cells.
Alternate Names	PMEF's Neo Resistant - Irradiated
背景信息	The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

应用
应用	The PMEF feeders are mitotically inactivated by treatment with irradiation & supplied at passage 3. These fibroblasts can be directly plated onto gelatin coated (Cat. No. ES-006-B) culture vessels.
主要应用	Stem Cell Culture

生物信息
细胞系类型	Primary Mouse Embryo Fibroblasts

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

储存和货运信息
存储条件	After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.