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纯度 ≥97% by SDS-PAGE 描述 概述 Recombinant, rat glial derived neurotrophic factor expressed in S. frugiperda insect cells. Native GDNF exists as a disulfide homodimeric glycoprotein and is a novel member of the TGF-β superfamily. Titrate the protein for optimal results in individual systems. Immbolized rrGFRa/Fc at 2 µg/ml (100 µl/neu) can bind GDNF at a linear range of 0.1-10 ng/ml. 产品目录编号 PF039 品牌系列 Calbiochem®
同义词 rrGDNF, Glial Derived Neurotrophic Factor 参考 参考 Durbec, P., et al. 1996. Nature 381, 789.
Jing, s., et al. 1996. Cell 85, 1113.
Treanor, J.J.S., et al. 1996. Nature 382 80.
Trupp, M., et al. 1996. Nature 381, 785.
Hudson, J., et al. 1995. Brain Res. Bull. 36, 425.
Tomac, A., et al. 1995. Nature 373, 335.
Lin, L.-F.H., et al. 1993. Science 260, 1130.
产品信息 形式 Lyophilized 配方 Lyophilized from 35% Acetonitrile, 0.1% TFA, 50 µg BSA/µg GDNF. 生物信息 生物活性 ED₅₀ = 1-3 ng/ml as measured by the ability to support the survival and stimulate neurite outgrowth of cultured embryonic chick dorsal root ganglia. Immbolized rrGFRα/Fc at 2 µg/ml (100 µl/neu) can bind GDNF at a linear range of 0.1-10 ng/ml. 纯度 ≥97% by SDS-PAGE 理化信息 污染物 Endotoxin: ≤1 EU/µg GDNF 溶解度 Sterile PBS containing ≥0.1% HSA or BSA 储存和货运信息 货运代码 Dry Ice Only
毒性 Standard Handling
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C.
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