merck millipore,默克密理博,PF036,Annexin V-Biotin Apoptosis Detection Kit

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merck millipore,默克密理博,PF036,Annexin V-Biotin Apoptosis Detection Kit
产品名称：Annexin V-Biotin Apoptosis Detection Kit
产品型号：PF036

merck millipore,默克密理博,PF036,Annexin V-Biotin Apoptosis Detection Kit

产品介绍

merck millipore,默克密理博,PF036,Annexin V-Biotin Apoptosis Detection Kit

重要规格表

检测方法
Fluorescence

描述
概述	Novel RAPID™ protocol and easy sample preparation. Optimized for flow cytometry and fluorescence microscopy applications. Useful for detection of membrane changes during apoptosis. Also useful in conjugation with GFP or other fluorophores.
概述	User provides appropriately labeled streptavidin.
产品目录编号	PF036
品牌系列	Calbiochem®

参考
参考	Darzynkiewicz, Z., et al. 1997. Cytometry 27, 1. Frey, T. 1997. Cytometry 28, 253. Boersma, A.W.M., et al. 1996. Cytometry 24, 123. White, E. 1996. Genes and Devel. 10, 1. Martin, S.J., et al. 1995. J. Exp. Med. 182, 1545. Koopman, G., et al. 1994. Blood 84, 1415. Wyllie, A.H. 1993. Br. J. Cancer 67, 205. Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795. Fadok, V.A., et al. 1992. J. Immunology 148, 2207. Schmid, I., et al. 1992. Cytometry 13, 204. Kerr, J.F.R., et al. 1972. Cancer 26, 239.

参考

Darzynkiewicz, Z., et al. 1997. Cytometry 27, 1.
Frey, T. 1997. Cytometry 28, 253.
Boersma, A.W.M., et al. 1996. Cytometry 24, 123.
White, E. 1996. Genes and Devel. 10, 1.
Martin, S.J., et al. 1995. J. Exp. Med. 182, 1545.
Koopman, G., et al. 1994. Blood 84, 1415.
Wyllie, A.H. 1993. Br. J. Cancer 67, 205.
Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
Fadok, V.A., et al. 1992. J. Immunology 148, 2207.
Schmid, I., et al. 1992. Cytometry 13, 204.
Kerr, J.F.R., et al. 1972. Cancer 26, 239.

产品信息
检测方法	Fluorescence
声明	Sold under license of U.S. Patent 5,834,196.
形式	100 Tests
格式	Flow cytometry or fluorescence microscopy
试剂盒包含	Annexin V-biotin, 5X Binding Buffer, Propidium Iodide, RAPID™ Media Binding Reagent, and a user protocol.
正控制	Apoptotic Jurkat cells (dexamethasone or anti-Fas treated)

应用
主要应用	Flow Cytometry Immunofluorescence

生物信息
化验时间	15 - 30 min
样品类型	Intact cells
品种	a broad range of species

产品使用声明
预定用途	The Calbiochem^® Annexin V-Biotin Apoptosis Detection Kit is a non-isotopic system for the identification of cell membrane alterations that accompany programmed cell death. Detection may be performed by flow cytometry or by fluorescence microscopy using various fluorescent streptavidin or anti-biotin conjugates.

储存和货运信息
货运代码	Blue Ice Only
毒性	Multiple Toxicity Values, refer to MSDS
储存	+2°C to +8°C
存储条件	Annexin V-BIOTIN kit components are shipped on gel ice pack. Upon receipt, store kit at 4°C. To avoid reagent loss in tube caps, briefly pulse spin all small tubes before removing caps. See Precautions and Recommendations.
无需冷冻	Yes