merck millipore,默克密理博,AB3579,Anti-BimEL (pS69) human / (pS65) rat Antibody

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merck millipore,默克密理博,AB3579,Anti-BimEL (pS69) human / (pS65) rat Antibody
产品名称：Anti-BimEL (pS69) human / (pS65) rat Antibody
产品型号：AB3579
Anti-BimEL (pS69) human / (pS65) rat Antibody is an antibody against BimEL (pS69) human / (pS65) rat for use in WB. Less<<

merck millipore,默克密理博,AB3579,Anti-BimEL (pS69) human / (pS65) rat Antibody

产品介绍

merck millipore,默克密理博,AB3579,Anti-BimEL (pS69) human / (pS65) rat Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H	WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB3579
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-BimEL (pS69) human / (pS65) rat Antibody
背景信息	Bim (bcl-2-interacting mediator of cell death) is a proapoptotic member of the Bcl-2 family that shares only the BH3 domain with this family. There are three isoforms of Bim: Bim_EL, Bim_L, and Bim_S. Bim is involved in regulating the intrinsic mitochondrial apoptotic pathway by inducing cytochrome c release, which in turn, activates caspase-9 and then caspase-3. Bim also plays a critical role in central and peripheral deletion of T lymphocytes and in controlling B cell homeostasis and activation. Bim_EL, the long isoform of Bim, is a ~28 kDa protein that is predominantly expressed in T and B cells and is activated by ERK1/2 pathway. The activation of Bim_EL by ERK1/2 promotes its phosphorylation on serine 65 (serine 69 in human), targeting it for degradation via the proteasome.

产品信息
格式	Affinity Purified
控制	Hek293T co-expressing rat Bim_EL and active MEKK1 (MEKK1δ).
演示	Dulbecco's phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide

应用
应用	Anti-BimEL (pS69) human / (pS65) rat Antibody is an antibody against BimEL (pS69) human / (pS65) rat for use in WB.
主要应用	Western Blotting
应用说明	For Western blotting applications, we recommend using the antibody at a 1:1000 dilution.

生物信息
免疫原品种	The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of rat Bim_EL that contains serine 65 (serine 69 in the human sequence). The sequence is conserved in human and mouse.
宿主	Rabbit
特异性	Rat Bim_EL when phosphorylated at serine 65. Human Bim_EL when phosphorylated at serine 69. Mouse Bim_EL (100% conserved) has not been tested, but is expected to react.
品种反应性	RatHuman
Species Reactivity Note	Mouse BimEL (100% homologous) has not been tested, but is expected to react.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_207002.2 NM_138622.2 NM_138623.2 NM_138624.2 NM_138626.2 NM_138627.2 NM_207003.1 NM_006538.3 NM_138621.3
Entrez基因汇总	The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified.
基因符号	BIM-alpha6 BCL2L11 BIM BOD BimEL BIM-beta6 BimL BIM-beta7 BAM
修改	Phosphorylation
UniProt编号	O43521
UniProt汇总	FUNCTION: SwissProt: O43521 # Induces apoptosis. Isoform BimL is more potent than isoform BimEL. SIZE: 198 amino acids; 22171 Da SUBUNIT: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including Mcl-1, Bcl-2, Bcl-X(L), BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK (By similarity). SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein. Note=Associated with intracytoplasmic membranes (By similarity). DOMAIN: SwissProt: O43521 The BH3 motif is required for Bcl-2 binding and cytotoxicity. SIMILARITY:

产品使用声明
质量保证	Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bim. The final product is generated by affinity chromatography using a Bim_EL-derived peptide that is phosphorylated at serine 65 (serine 69 in the human sequence).
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bim. The final product is generated by affinity chromatography using a Bim_EL-derived peptide that is phosphorylated at serine 65 (serine 69 in the human sequence).

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.