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可用的分析物 品种反应性 主要应用 检测方法 α-Synuclein H, M, R ELISA Chromogenic 描述 产品目录编号 NS400 品牌系列 Chemicon®
商名 - Chemicon
描述 α-Synuclein ELISA Kit 概述 Background
α-Synuclein, a member of the synuclein family which also includes α- and γ-synuclein, is a small cytoplasmic protein predominately expressed in neural tissue, particularly in the neocortex, hippocampus, striatum, thalamus, and cerebellum. Though its normal physiological function is unclear, α-Synuclein is highly enriched at the presynaptic terminals and may have a role in membrane associated processes at the presynaptic terminal. It is specifically up regulated in presynaptic terminals of discrete neuronal populations in songbird brain during a period of song-acquisition-related synaptic rearrangement (George et al. 1995). Mice deficient in α-Synuclein display enhanced dopamine release in the nigrostriatal dopamine system suggesting that α-synuclein is a negative regulator of dopamine neurotransmission (Abeliovich et al. 2000). Furthermore, knockdown of α-synuclein in cultured hippocampal neurons results in a decline in the distal pool of presynaptic vesicles (Murphy et al. 2000). Also, purified α-Synuclein inhibits phospholipase D2 mediated hydrolysis of phosphatidylcholine to phosphatidic acid (Jenco et al. 1998) which may play a role in cytoskeletal reorganization and endocytosis at the plasma membrane (Colley et al. 1997). Thus, α-Synuclein may serve to integrate presynaptic signaling and membrane trafficking.
Dysfunctional regulation in α-Synuclein is strongly implicated as a critical factor in several neurodegenerative diseases. Misfolding, aggregation and fibrillation are associated with the pathogenesis of Parkinson disease (Schulz 2008). α-Synuclein peptides are also a major component of amyloid plaques in Alzheimer's disease (Allsop et al. 2008). It has also been identified in the Glial Cytoplasmic Inclusions found in Multiple System Atrophy (Yoshida 2007).
Test Principle
The α-Synuclein ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of α-Synuclein protein in whole cell or tissue extracts (Figure 1). The kit utilizes a 96-well microtiter plate that is pre-coated with an α-Synuclein specific capture antibody. A test sample or the protein standard provided in the kit is then added to each test well and incubated allowing for α-Synuclein antigen to be captured. The plate is subsequently washed to remove any unbound non-specific material and then incubated with a detection antibody that is specific to α-Synuclein. Any unbound detection antibody is washed away prior to a final incubation with HRP conjugated secondary antibody. After the addition of TMB substrate and stop solution, the absorbance is measured at 450 nm using a plate reader. This enzymatic detection format allows for sensitive and specific detection of α-Synuclein protein in test samples.
The entire assay takes less than 5 hours to complete with minimal hands-on time. For ease of use many of the reagents are supplied in a ready-to-use formulation. The kit also includes an α-Synuclein protein standard that is run as a positive control to develop a standard curve.
Alternate Names - α-Synuclein
产品信息 部件 - Stop Solution: One 12 mL bottle of an HCl solution (Ready to Use).
- 96-well α-Synuclein Assay Plate
- 5X Lysis Buffer / ELISA Diluent: 20mL
- 10X Wash Buffer: 130mL
- α-Synuclein Proein Standard (1 mg/mL): 10µL
- Detection Antibody: anti-αsynuclein: 10µL
- HRP Conjugated Secondary Antibody: 10µL
- TMB/E Solution: 10 mL bottle of a Ready to Use solution of 3,3',5,5'-tetramethylbenzidine in a proprietary buffer with enhancer.
检测方法 Chromogenic 应用 应用 This α-Synuclein ELISA Kit is used to measure & quantify α-Synuclein levels in Neuroscience research. 主要应用 - ELISA
生物信息 品种反应性 HumanMouseRat 可用的分析物 - α-Synuclein
Entrez基因编号 - NM_000345.2
Entrez基因汇总 Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Two alternatively spliced transcripts of SNCA have been identified. Additional splicing may be present but the full-length nature of these variants has not been determined. 基因符号 - Alpha-synuclein
- SNCA
- PD1
- alpha-synuclein
- NACP
- PARK4
- PARK1
- MGC110988
UniProt编号 - P37840
UniProt汇总 FUNCTION: SwissProt: P37840 # May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Induces fibrillization of microtubule- associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation.
SIZE: 140 amino acids; 14460 Da
SUBCELLULAR LOCATION: Cytoplasm. Membrane. Nucleus. Note=Membrane- bound in dopaminergic neurons. Also found in the nucleus.
TISSUE SPECIFICITY: Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
PTM: Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress. & Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers. & Ubiquitinated. The predominant conjugate is the diubiquitinated form (By similarity).
SIMILARITY: Belongs to the synuclein family.理化信息 灵敏度 - 3 ng/mL
标准曲线范围 - 3 to 60 ng/mL
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Storage: The α-Synuclein ELISA Kit is a dual temperature storage kit. Upon receipt, the α-Synuclein protein standard and HRP conjugated secondary antibody should be stored at -20ºC. All other kit components should be stored at 4ºC.
Stability: Kit components are stable for a minimum of 4 months from the date of receipt if stored and handled as described above.
包装信息 数量 1 kit 包装 96 wells
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