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品种 来源 纯度 Mouse Mouse submaxillary gland (Darling & Shooter, 1984). 97% pure as determined by SDS-PAGE. 描述 产品目录编号 NC010 品牌系列 Chemicon®
商名 - Chemicon
描述 Nerve Growth Factor 7.0s 概述 MOLECULAR WT.:
130-140 kD. 7S NGF is composed of 3 subunits; 1 beta subunit (2.5S), 2 alpha subunits and 2 gamma subunits.Alternate Names - holo-NGF
背景信息 In the mouse submaxillary gland the neurotrophically active NGF, beta-NGF (2.5S), is part of a larger protein complex, 7S-NGF, which has the stoichiometry alpha2betagamma2 (+ 1 or 2 zinc ions) where the alpha and gamma subunits are members of the glandular kallikrein family of serine proteases, each of about 26 000 kDa molecular weight. The alpha and gamma subunits actually inhibit beta-NGF action and must be dissociated for biological activity to be observed.
This 7S complex has not been shown to occur in other mouse tissues, or in other species, probably because the alpha and gamma subunits, which are members of a larger glandular kallikrein family, apparently have a very restricted expression. The alpha and gamma subunits, members of the serine proteinase family, have quite similar amino acid sequences, although only the gamma subunit is catalytically active. The alpha subunit has undergone several mutations that preclude this function, including loss of the cleavage site needed for processing from the zymogen to the active form.产品信息 演示 Lyophilized. NC010 should be reconstituted with sterile DMEM at a concentration of
10 μg/mL and stored at -70°C. Medium with mNGF cannot be reused due to mNGF degradation.生物信息 浓缩 1 mg/mL 纯度 97% pure as determined by SDS-PAGE. 来源 Mouse submaxillary gland (Darling & Shooter, 1984). 品种 Mouse 特异活性 BIOLOGICAL ASSAY: <br /><br />The neurotrophic activity is in the range of 0.5-50 ng/mL using rat PC12 cells cultured for 7-14 days. The recommended concentration to be used in vitro for maintenance of sympathetic and immature sensory nerve cells is 50-100 ng/mL medium (Varon et al., 1967). 基因符号 - NGFA
- KLK1B4
- K1KB4
- KLK4
UniProt编号 - P00757
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µg
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