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品种反应性 主要应用 宿主 格式 抗体类型 H, M ICC, WB Rb Diluted Culture Supernatant Monoclonal Antibody 描述 产品目录编号 MABT146 描述 Anti-CD97 Antibody, clone EPR4427, Rabbit | MABT146 Alternate Names - seven-transmembrane, heterodimeric receptor associated with inflammation
- seven-span transmembrane protein
- seven transmembrane helix receptor
- Leukocyte antigen CD97
- CD97 antigen
- CD97 molecule
背景信息 CD97 is a member of the EGF-TM7 family of class II seven-span transmembrane (7-TM) molecules. It is a glycoprotein that is present on the surface of most activated leukocytes and spans the membrane seven times, which is a defining feature of G protein-coupled receptors. CD97 has an extended extracellular region with several N-terminal epidermal growth factor (EGF)-like domains, which mediate binding to its cellular ligand, decay accelerating factor (DAF, CD55), a regulatory protein of the complement cascade. The presence of structural features characteristic of extracellular matrix proteins and transmembrane proteins suggests that this protein is a receptor involved in both cell adhesion and signaling processes early after leukocyte activation. 产品信息 格式 Diluted Culture Supernatant 控制 - Human tonsil, Jurkat, U937, and fetal thymus tissue lysates
演示 Rabbit Monoclonal in buffer containing 50% Glycerol, 0.01% sodium azide and 0.05% BSA. 应用 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: 1:250-1:500 dilution from a representative lot was used in IC.
Immunoprecipitation Analysis: 1:10 to 1:100 dilution from a representative lot was used in IP.
Immunohistochemistry Analysis: 1:250-1:500 dilution from a representative lot detected CD97 in human spleen tissue.生物信息 免疫原品种 Synthetic peptide corresponding to human CD97 at the extracellular domain 表位 Extracellular domain 克隆 EPR4427 宿主 Rabbit 同种型 IgG 品种反应性 HumanMouse Species Reactivity Note Demonstrated to react with Human and Mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - P48960
基因符号 - TM7LN1
纯化方法 Unpurified UniProt编号 - P48960
UniProt汇总 FUNCTION: Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration By similarity.
SUBUNIT STRUCTURE: Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit). Interacts with complement decay-accelerating factor (DAF). The largest isoform 1) interacts with chondroitin sulfate.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CD97 antigen subunit alpha: Secreted › extracellular space.
TISSUE SPECIFICITY: Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid.
INDUCTION: Rapid up-regulation during lymphocyte activation.
DOMAIN: The first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.
Binding to chondroitin sulfate is mediated by the fourth EGF domain.
PTM: Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit By similarity.
SEQUENCE SIMILARITIES: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 5 EGF-like domains.
Contains 1 GPS domain.
SEQUENCE CAUTION: The sequence AAC27673.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence BAC06178.1 differs from that shown. Reason: Erroneous gene model prediction.产品使用声明 质量保证 Evaluated by Western Blot in human tonsil, Jurkat, U937, and fetal thymus tissue lysates.
Western Blot Analysis: 1:1,000-1:10,000 dilution of this antibody detected CD97 in human tonsil, Jurkat, U937, and fetal thymus tissue lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. 包装信息 数量 100 µL
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