merck millipore,默克密理博,MABT144,Anti-Alpha-Actinin 4 Antibody, clone EPR2533(2), rabbit monoclonal

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merck millipore,默克密理博,MABT144,Anti-Alpha-Actinin 4 Antibody, clone EPR2533(2), rabbit monoclonal
产品名称：Anti-Alpha-Actinin 4 Antibody, clone EPR2533(2), rabbit monoclonal
产品型号：MABT144
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Alpha-Actinin 4 Antibody, clone EPR2533(2) More>>

merck millipore,默克密理博,MABT144,Anti-Alpha-Actinin 4 Antibody, clone EPR2533(2), rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABT144,Anti-Alpha-Actinin 4 Antibody, clone EPR2533(2), rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Diluted Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	MABT144
描述	Anti-Alpha-Actinin 4 Antibody, clone EPR2533(2), rabbit monoclonal
Alternate Names	actinin alpha4 isoform actinin, alpha 4 Non-muscle alpha-actinin 4 F-actin cross-linking protein ACTININ-4 alpha-actinin-4
背景信息	Alpha-actinin is a ubiquitously expressed cytoskeletal protein found in numerous actin structures. It consists of an N-terminal actin-binding domain, a central rod domain, and a C-terminal domain; the protein functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinin 4 is a non-muscle isoform version of alpha-actinin that is concentrated in the cytoplasm (2). It plays a role in cell motility, invasion, and metastasis of cancer cells (3). Alpha-actinin 4 is commonly expressed in filopodia, lamellipodia, stress fibers, and sites of adhesion (4).

产品信息
格式	Diluted Culture Supernatant
控制	Human fetal skeletal muscle tissue lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Alpha-Actinin 4 Antibody, clone EPR2533(2), Rabbit is validated for use in WB, IC, IH(P), IP for the detection of Alpha-Actinin 4.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot detected Alpha-Actinin 4 in HeLa cells. Immunohistochemistry Analysis: A 1:100-250 dilution from a representative lot detected Alpha-Actinin 4 in human kidney tissue. Immunoprecipitation Analysis: A 1:10-100 dilution from a representative lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to human Alpha-Actinin 4 at the N-terminus.
表位	N-terminus
克隆	EPR2533(2)
宿主	Rabbit
特异性	This antibody recognizes Alpha-Actinin 4 at the N-terminus.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Demonstrated to react with human, mouse, and rat.
抗体类型	Monoclonal Antibody
基因符号	DKFZp686K23158 FSGS FSGS1
纯化方法	Unpurified
UniProt编号	O43707
UniProt汇总	FUNCTION: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. SUBUNIT STRUCTURE: Homodimer; antiparallel By similarity. Binds TRIM3 at the N-terminus By similarity. Identified in a complex with CASK, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1 By similarity. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts with BAIAP1 and PDLIM2. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF7, oral floor cancer IMC2, and bladder cancer KU7). TISSUE SPECIFICITY: Widely expressed. INVOLVEMENT IN DISEASE: Defects in ACTN4 are the cause of focal segmental glomerulosclerosis type 1 (FSGS1) [MIM:603278]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. SEQUENCE SIMILARITIES: Belongs to the alpha-actinin family. Contains 1 actin-binding domain. Contains 2 CH (calponin-homology) domains. Contains 2 EF-hand domains. Contains 4 spectrin repeats. SEQUENCE CAUTION: The sequence AAC17470.1 differs from that shown. Reason: Frameshift at positions 19, 26, 124, 130, 589, 594, 787 and 806. The sequence BAA24447.1 differs from that shown. Reason: Erroneous initiation.

产品使用声明
质量保证	Evaluated by Western Blot in human fetal skeletal muscle tissue lysate, A431, MCF-7 and HeLa cell lysates. Western Blot Analysis: A 1:1,000 to 1:10,000 dilution of this antibody detected Alpha-Actinin 4 in human fetal skeletal muscle tissue lysate, A431, MCF-7 and HeLa cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing Solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below
-20°C may cause glycerol containing Solutions to become frozen during storage.