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品种反应性 主要应用 宿主 格式 抗体类型 Lz, H, Ch, R IHC, WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MABT121 描述 Anti-Vimentin Antibody, clone 3CB2 Alternate Names - Vimentin
背景信息 Vimentin is a class III intermediate filament protein (homopolymers) found in various non-epithelial cells including mesenchymal cells and fibroblasts. In many cells of mesenchymal origin, it is one of the most prominent phosphoproteins, especially during cell division when enhanced phosphorylation of vimentin corresponds with the reorganization of vimentin filaments. 产品信息 格式 Purified 控制 - ARPE cell lysate
演示 Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide. 应用 应用 Anti-Vimentin Antibody, clone 3CB2 is an antibody against Vimentin for use in WB, IC, IH. 主要应用 - Immunohistochemistry
- Western Blotting
- Immunocytochemistry
应用说明 Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in human central and peripheral retinal sections (Perez-Alvarez, M.J., et al. (2008). J Neurosci Res. 86(8):1871-1883).
Western Blot Analysis:
A representative lot was used to detect vimentin in chicken and rat total brain extracts (Prada, FA., et al. (1995). GLIA 15:389-400).
Immunohistochemistry Analysis:
A representative lot was used to detect vimentin in E3 chick embryo sections and different regions of the developing chick central nervous system (Prada, FA., et al. (1995). GLIA 15:389-400).生物信息 免疫原品种 Vimentin extracted from chicken embryonic brain tissue. 克隆 3CB2 宿主 Mouse 同种型 IgMκ 品种反应性 LizardHumanChickenRat Species Reactivity Note Demonstrated to react with Human.
Demonstrated to react with Chameleon and Rat based on the following reference:
GLIA 15:389-400 (1995), Prada, F. A., et. al.
Predicted to react to Chicken based on immunogen design.抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001041541
基因符号 - VIM
纯化方法 Ion Exchange UniProt编号 - P09654
UniProt汇总 FUNCTION: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
SUBUNIT STRUCTURE: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM.产品使用声明 质量保证 Evaluated by Western Blot in ARPE19 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Vimentin in ARPE19 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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