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品种反应性 主要应用 宿主 格式 抗体类型 H, R, M WB M Purified Monoclonal Antibody 描述 产品目录编号 MABS95 描述 Anti-Wnt-1 Antibody, clone 20C3.1 Alternate Names - Proto-oncogene Int-1 homolog
- Proto-oncogene Wnt-1
背景信息 Wnt-1 was first identified as a proto-oncogene activated by viral insertion in mouse mammary tumors. The Wnt-1 gene encodes a member of a large family of secreted proteins that are cysteine-rich, glycosylated, and poorly soluble. Wnt-1 serves as a ligand for the RYK coreceptor, resulting in Wnt signaling. The Wnt signalling pathway mediates the levels of cytosolic beta-catenin, a multi-functional protein that associates with membrane-bound E-cadherin as well as several DNA-binding proteins, such as members of the TCF/LEF family. Additionally, it serves a ligand for members of the frizzled family of seven transmembrane receptors and plays a role in development of the central nervous system. 产品信息 格式 Purified 控制 - Mouse brain tissue lysate
演示 Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide. 应用 应用 Anti-Wnt-1 Antibody, clone 20C3.1 detects level of Wnt-1 & has been published & validated for use in WB. 主要应用 - Western Blotting
应用说明 Western Blot Analysis: A 1:2,000 dilution from a representative lot detected Wnt-1 in human brain tissue lysate. 生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human Wnt-1. 克隆 20C3.1 宿主 Mouse 同种型 IgMκ 品种反应性 HumanRatMouse Species Reactivity Note Demonstrated to react with Human, Rat, and Mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_005421
Entrez基因汇总 The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. 基因符号 - INT1
- WNT1
纯化方法 Purified by ion exchange chromatography UniProt编号 - P04628
UniProt汇总 FUNCTION: Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
SUBUNIT STRUCTURE: Interacts with PORCN. Interacts with RSPO1, RSPO2 and RSPO3. Interacts with WLS.
SUBCELULLAR LOCATION: Secreted > extracellular space > extracellular matrix.产品使用声明 质量保证 Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected Wnt-1 in 10 µg of mouse brain tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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