merck millipore,默克密理愽
  • merck millipore,默克密理博,MABS63,Anti-phospho-PLCγ-1 (Tyr783) Antibody, clone 12F4.2

  • merck millipore,默克密理博,MABS63,Anti-phospho-PLCγ-1 (Tyr783) Antibody, clone 12F4.2

    产品名称：Anti-phospho-PLCγ-1 (Tyr783) Antibody, clone 12F4.2
    产品型号：MABS63
    Anti-phospho-PLCγ-1 (Tyr783) Antibody, clone 12F4.2 detects level of phospho-PLCγ-1 (Tyr783) & has been published & validated for use in WB, FC.

    merck millipore,默克密理博,MABS63,Anti-phospho-PLCγ-1 (Tyr783) Antibody, clone 12F4.2

  • 产品介绍
  • merck millipore,默克密理博,MABS63,Anti-phospho-PLCγ-1 (Tyr783) Antibody, clone 12F4.2

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HFC, WBMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MABS63
    描述 Anti-phospho-PLCγ-1 (Tyr783) Antibody, clone 12F4.2
    Alternate Names
    • phospholipase C-148
    • phospholipase C, gamma 1
    • 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
    • monophosphatidylinositol phosphodiesterase
    • triphosphoinositide phosphodiesterase
    • phosphatidylinositol phospholipase C
    • inositoltrisphosphohydrolase
    • phospholipase C, gamma 1 (formerly subtype 148)
    • phosphoinositidase C
    • Phosphoinositide phospholipase C
    • PLC-gamma-1
    • Phospholipase C-gamma-1
    • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
    背景信息 Phospholipase C (PLC) is a group of enzymes that play a critical role in signal transduction during the hydrolysis of membrane phospholipids as a response to extracellular stimuli. Part of the phosphinositide-specific PLC family, PLC У-1 is known for its involvement in growth-factor-dependent signal transduction. PLC У-1 is made up of 2 SH2 domains, one SH3 domain and 2 tyrosine phophrylation sites. Almost all growth factors can stimulate tyrosine phosphorylation and subsequent activation of PLC У-1. (Tvorogov, 2005). Of the protein kinases that stimulate tyrosine phosphorylation, PLC У-1 is mostly dependent on three different kinds; ZAP-10, Lck, and Itk. (Kang, 2003). Activation of PLC У-1 catalyzes the hydrolysis of phosphatidylinositol 4,5-biphosphate which in turn produces two second messengers, diacylglycerol (DAG) and inositol 1,4,5-triphosphate(IP3). DAG serves to activate protein Kinase 3, while IP3 mediates the release of calcium. PLC У-1 is crucial in its role to the mediation of cellular proliferation and differentiation.
    产品信息
    格式 Purified
    控制
    • Untreated and EGF treated A431 cell lysates
    演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
    应用
    应用 Anti-phospho-PLCγ-1 (Tyr783) Antibody, clone 12F4.2 detects level of phospho-PLCγ-1 (Tyr783) & has been published & validated for use in WB, FC.
    主要应用
    • Flow Cytometry
    • Western Blotting
    应用说明 Flow Cytometry Analysis: 1 µg from a previous lot detected PLCγ-1 in EGF treated A431 cells.
    生物信息
    免疫原品种 Linear peptide corresponding to human PLCγ-1 phosphorylated at Tyr783.
    表位 Phosphorylated Tyr783
    克隆 12F4.2
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 This antibody recognizes PLCγ-1 phosphorylated at Tyr783.
    同种型 IgG1κ
    品种反应性 Human
    Species Reactivity Note Demonstrated to react with human.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_002651.2
    Entrez基因汇总 The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
    基因符号
    • PLC-148
    • PLC-II
    • PLC1
    • PLCgamma1
    • PLC148
    • NCKAP3
    修改
    • Phosphorylation
    纯化方法 Protein G purfied
    UniProt编号
    • P19174
    UniProt汇总 FUNCTION: PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.

    CATALYTIC ACTIVITY: 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    COFACTOR: Calcium.

    SUBUNIT STRUCTURE: Interacts with AGAP2 via its SH3 domain By similarity. Interacts with phosphorylated LAT upon TCR activation. Interacts with the Pro-rich domain of TNK1 via its SH3 domain. Associates with BLNK, VAV1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB in activated T-cells; which inhibits phosphorylation. Interacts with SHB. Interacts via its SH3 domain with the Arg/Gly-rich-flanked Pro-rich domains of KHDRBS1/SAM68. This interaction is selectively regulated by arginine methylation of KHDRBS1/SAM68. Interacts with INPP5D/SHIP1, THEMIS and CLNK By similarity. Interacts with RALGPS1. Interacts (via SH3 domain) with HEV ORF3 protein.

    DOMAIN: The SH3 domain mediates interaction with CLNK By similarity. The SH3 domain also mediates interaction with RALGPS1.

    PTM: The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors.

    Ubiquitinated by CBLB in activated T-cells by similarity.

    SEQUENCE SIMILARITIES: Contains 1 C2 domain. Contains 1 EF-hand domain. Contains 2 PH domains. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain. Contains 2 SH2 domains. Contains 1 SH3 domain.
    产品使用声明
    质量保证 Evaluated by Western Blot in untreated and EGF treated A431 cell lysates.

    Western Blot Analysis: 0.5 µg/mL of this antibody detected PLCγ-1 on 10 µg of untreated and EGF treated A431 cell lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MABS63,Anti-phospho-PLCγ-1 (Tyr783) Antibody, clone 12F4.2

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