merck millipore,默克密理博,MABS276,Anti-pan polyglycylated Tubulin Antibody, clone AXO 49

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
A Broad Range Of Species, Dr, Po, Sn, Su, M	WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	MABS276
描述	Anti-pan polyglycylated Tubulin Antibody, clone AXO 49
背景信息	Several post-translational modifications of tubulin have been identified in eukaryotic cells. Glycylation is a poly-modification which occurs as lateral branching of glycine chains of variable length identified in axonemal tubulin of Paramecium cilia. This modification has been detected on tubulin and/or cilia/flagella of many unicellular and pluricellular organisms. The differential distribution of distinct polyglycylated tubulin isoforms between cytoplasmic and axonemal compartments indicates that polyglycylation levels of tubulin is highly regulated at the cell level. The AXO49 antibody is reactive mainly on axonemal tubulin of motile cilia and flagella which are polyglycylated. In some cases, reactivity may also be observed on other very stable microtubule networks. This antibody is useful for cilia and flagella detection.

产品信息
格式	Purified
演示	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	Detect Tubulin using this mouse monoclonal antibody, Anti-pan polyglycylated Tubulin Antibody, clone AXO 49 validated for use in western blotting, Immunofluorescence & Dot Blot.
主要应用	Western Blotting
应用说明	Immunofluorescence Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in mouse spermatozoa (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.). Dot Blot Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in synthetic monoglycylated tubulin peptides (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.). Western Blot Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in a panel of total protein extracts from select species (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.). Immunoflourescence Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in lemur, human, and sea urchin spermatozoa (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).

生物信息
免疫原品种	Insoluble fraction of Paramecium cilia (Paramecium axonemes)
克隆	AXO 49
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	This antibody recognizes the lateral connecting branches of a polymer, which contains at least 3 glycine residues. This antibody demonstrates cross reactivity against polyglycylated tubulin, as well as with other polyglycylated proteins .
同种型	IgG1κ
品种反应性	A Broad Range Of SpeciesDrosophilaPigSnailSea UrchinMouse
Species Reactivity Note	Demonstrated to react with Apis, Mouse, Drosophila, Sea Urchin, Paramecium and other protists, Porcine, Lemur, Snail, Trout, Ram, Sea Urchin. Negative on Euglena and human cilia.
抗体类型	Monoclonal Antibody
纯化方法	Protein G purified

产品使用声明
质量保证	Evaluated by Western Blotting on total cytoskeletal proteins of Paramecium. : A 1:50,000 dilution of this antibody detected polyglycylated Tubulin in 10 µg of Paramecium total cytoskeletal proteins.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt.

包装信息
数量	50 µL

merck millipore,默克密理博,MABS276,Anti-pan polyglycylated Tubulin Antibody, clone AXO 49

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