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品种反应性 主要应用 宿主 格式 抗体类型 H WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MABS133 描述 Anti-CHIP Antibody, clone 2F2.1 Alternate Names - STIP1 homology and U box-containing protein 1
- E3 ubiquitin-protein ligase CHIP
- Antigen NY-CO-7
- CLL-associated antigen KW-8
- Carboxy terminus of Hsp70-interacting protein
背景信息 Carboxyl-terminus of Hsc70 interacting protein (CHIP) is an E3 ubiquitin ligase. It has been detected in the heart, pancreas, brain, placenta, liver, lung, kidney, and skeletal muscle. CHIP is a multidomain protein containing an N-terminal TPR domain and a C-terminal U box domain both of which may interact with chaperones such as Hsc70 and Hsp70. CHIP is mainly involved with recognizing and marking misfolded proteins for degradation; however, CHIP is a unique E3 protein in requiring chaperone association with target proteins before marking them for ubiquitination. CHIP is therefore important for cell survival as it prevents the accumulation of non-functional proteins in the cell which could potentially produce cytotoxic effects. Previous studies have also suggested that CHIP may be involved in Toll-like receptor (4 and 6) signaling by activating downstream targets such as Src and PKCζ. 产品信息 格式 Purified 控制 - Human kidney tissue lysate
演示 Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide. 应用 应用 Anti-CHIP Antibody, clone 2F2.1 detects level of CHIP & has been published & validated for use in IHC, Western Blotting. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry Analysis: A 1:50 dilution from a representatative lot detected CHIP in human placenta tissue. 生物信息 免疫原品种 GST-tagged recombinant protein corresponding to mouse CHIP. 克隆 2F2.1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgMκ 品种反应性 Human Species Reactivity Note Demonstrated to react in human. Predicted to react with mouse based on immunogen design. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_005852
Entrez基因汇总 STUB1, or CHIP, is a ubiquitin ligase/cochaperone that participates in protein quality control by targeting a broad range of chaperone protein substrates for degradation. 基因符号 - PP1131
- STUB1
- CHIP
纯化方法 Purifed by ion-exchange chromatography UniProt编号 - Q9UNE7
UniProt汇总 FUNCTION: E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT STRUCTURE: Homodimer. Interacts with BAG2, and with the E2 ubiquitin conjugating enzymes UBE2D1, UBE2D2 and UBE2D3. Interacts with the C-terminal domains of HSPA8 and HSPA1A. Detected in a ternary complex containing STUB1, HSPA1A and HSPBP1. Interacts with MKKS. Interacts with DYX1C1 and POLB. Interacts (via TPR repeats) with HSP90AA1. Interacts (via the U-box domain) with the UBE2V2-UBE2N heterodimer; the complex has a specific 'Lys-63'-linked polyubiquitination activity.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
DOMAIN: The TPR domain is essential for ubiquitination mediated by UBE2D1.
POST-TRANSLATIONAL MODIFICATION: Phosphorylated upon DNA damage, probably by ATM or ATR. Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2.
MISCELLANEOUS: Antibodies against STUB1 are found in patients with chronic lymphocytic leukemia (CLL) and in colorectal cancer patients.
SEQUENCE SIMILARITIES: Contains 3 TPR repeats. Contains 1 U-box domain.产品使用声明 质量保证 Evaluated by Western Blot in human kidney tissue lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected CHIP in 10 µg of human kidney tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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