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品种反应性 主要应用 宿主 格式 抗体类型 M, R, H WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MABN72 描述 Anti-pan-MAGUK Antibody, clone K28/86 Alternate Names - Synapse-associated protein 90
- discs, large homolog 4 (Drosophila)
- discs large homolog 4
- disks large homolog 4
- Tax interaction protein 15
- post-synaptic density protein 95
- Postsynaptic density protein 95
背景信息 The MAGUKs are membrane-associated guanylate kinase related (MAGUK) proteins that are defined by their inclusion of PDZ, SH3 and GUK domains. The GUK domain that they have is structurally very similar to that of the guanylate kinases, which converts ATP into ADP. However, it is known to be catalytically inactive as the P-Loop which normally binds ATP is absent. It is thought that the MAGUKs have adopted the GUK domain for their own purposes, primarily based on its ability to form protein-protein interactions with cytoskeleton proteins, microtubule/actin based machinery and molecules involved in signal transduction. In addition many of the MAGUK proteins also contain regions homologous of CaMKII, WW and L27 domains. Some of the members of the MAGUK family of proteins include DLG4 (Disks large homolog 4), DLG2, PSD95, MPP1 and other cytoskeleton-interacting proteins. 产品信息 格式 Purified 控制 - Rat brain tissue lysate
演示 Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-pan-MAGUK Antibody, clone K28/86 is an antibody against pan-MAGUK for use in IH, WB & IP. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected the MAGUK family of proteins in rat cerebellum and rat hypothalamus tissues.
Immunoprecipitation Analysis: A previous lot of this antibody was successfully used by an independent laboratory in IP. (Rasband, M., et al. (2002). The Journal of Cell Biology. 159(4):663–672.)生物信息 免疫原品种 Recombinant protein corresponding to a fusion domain of human PSD95/SAP90 proteins. 克隆 K28/86 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody exhibits cross reactivity against recombinant Chapsyn-110, SAP97, and SAP102. (UC Davis/NIH NeuroMab Facility) 同种型 IgG1λ 品种反应性 MouseRatHuman Species Reactivity Note Demonstrated to react with rat. Predicted to react with human based on immunogen design. Predicted to react with mouse based on 100% sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001356
Entrez基因汇总 This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. 基因符号 - PSD-95
- DLG4
- SAP90
- PSD95
- SAP-90
纯化方法 Protein G UniProt编号 - P78352
UniProt汇总 FUNCTION: Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B (By similarity).
SUBUNIT STRUCTURE: Interacts with ANKS1B, KLHL17 and PRR7 (By similarity). Interacts through its PDZ domains with NETO1. Interacts through its first two PDZ domains with GRIN2A, GRIN2B, GRIN2C, GRIN2D, ACCN3, certain splice forms of GRIN1, KCND2, CXADR and SYNGAP1. Interacts through its second PDZ domain with the PDZ domain of NOS1 or the C-terminus of CAPON. May interact with HTR2A. Interacts through its guanylate kinase-like domain with DLGAP1/GKAP, DLGAP2, DLGAP3, DLGAP4, MAP1A and BEGAIN. Interacts through its third PDZ domain with CRIPT (By similarity). Interacts through its first two PDZ domains with KCNA1, KCNA2, KCNA3, KCNA4 and ERBB4. Interacts through its first PDZ domain with GRIK2, KCNA4 and CRIPT. Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. Interacts through its guanylate kinase-like domain with KIF13B. Isoform 2 interacts through an L27 domain with HGS/HRS and the first L27 domain of CASK. Interacts with LRFN1, LRFN2 and LRFN4. Interacts with ANO2, ADAM22 and LGI1 (By similarity). Interacts with FRMPD4 (via C-terminus). Interacts (via PDZ1 and PDZ2 domains) with LRRC4; LRRC4B and SEMA4C. Interacts (via guanylate kinase-like domain) with SIPA1L1 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Cell junction › synapse. Cell junction › synapse › synaptosome (By similarity). Note: High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
TISSUE SPECIFICTY: Brain.
DOMAIN: The PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to post-synaptic density.
PTM: Palmitoylation of isoform 1 is required for targeting to postsynaptic density (By similarity).
SEQUENCE SIMILARITIES: Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.产品使用声明 质量保证 Evaluated by Western Blot in rat brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected members of the MAGUK protein family on 10 µg of rat brain tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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