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品种反应性 主要应用 宿主 格式 抗体类型 M, R ICC, IHC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABN70 描述 Anti-Slo1 Antibody, clone L6/60 Alternate Names - Slo-alpha
- potassium large conductance calcium-activated channel, subfamily M, alpha member 1
- Slo homolog
- stretch-activated Kca channel
- Maxi K channel
- Drosophila slowpoke-like
- Calcium-activated potassium channel, subfamily M subunit alpha-1
- Slowpoke homolog
- BK channel
- calcium-activated potassium channel subunit alpha-1
- BKCA alpha
- BKCA alpha subunit
- K(VCA)alpha
- SLO-ALPHA
背景信息 Slo1 (Calcium-activated potassium channel subunit alpha-1) may not only be activated by membrane depolarization but may also be by an increase in cytosolic Ca2+. The concentration of cytosolic Mg2+ also activates this potassium channel. Found to play a part in the repolarization of the membrane potential due to the fact that it diminishes the excitatory voltage in the cell. 产品信息 格式 Purified 控制 - Rat brain membrane tissue lysate
演示 Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect Slo1 using this Anti-Slo1 Antibody, clone L6/60 validated for use in IH, WB & IC. 主要应用 - Immunocytochemistry
- Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Slo1 in rat cerebellum tissue.
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Misonou, H., et al. (2006). J Comp Neurol. 496(3):289–302.)生物信息 免疫原品种 Recombinant protein corresponding to the S9-S10 segments of mouse Slo1. 表位 S9-S10 segments 克隆 L6/60 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes S9-S10 segments of Slo1. 同种型 IgG2aκ 品种反应性 MouseRat Species Reactivity Note Demonstrated to react with rat. Predicted to react with mouse based on immunogen design. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_034740
基因符号 - Slo1
- SLO1
- KCa1.1
- SAKCA
- SLO
- k(VCA)alpha
- mSLO1
- BKTM
- MaxiK
- KCNMA1
- hSlo
- KCNMA3
纯化方法 Protein G UniProt编号 - Q08460
UniProt汇总 FUNCTION: Potassium channel activated by both membrane depolarization or increase in cytosolic Ca2+ that mediates export of K+. It is also activated by the concentration of cytosolic Mg2+. Its activation dampens the excitatory events that elevate the cytosolic Ca2+ concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca2+, caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).
ENZYME REGULATION: Ethanol and carbon monoxide-bound heme increase channel activation. Heme inhibits channel activation (By similarity).
SUBUNIT STRUCTURE: Homotetramer; which constitutes the calcium-activated potassium channel. Interacts with beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. Beta subunits are accessory, and modulate its activity.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (By similarity).
DOMAIN: The S0 segment is essential for the modulation by the accessory beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4.
The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor.
The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium.
The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel. It includes binding sites for Ca2+ and Mg2+.
The calcium bowl constitutes one of the Ca2+ sensors and probably acts as a Ca2+-binding site. There are however other Ca2+ sensors regions required for activation of the channel.
The heme-binding motif mediates inhibition of channel activation by heme. Carbon monoxide-bound heme leads to increased channel activation (By similarity).
PTM: Phosphorylated Probable. Phosphorylation by kinases such as PKA and/or PKG. In smooth muscles, phosphorylation affects its activity.
MISCELLANEOUS: The protein was initially thought to contain two functionally distinct parts: The core channel (from the N-terminus to the S9 segment) that mediates the channel activity, and the cytoplasmic tail (from the S9 segment to the C-terminus) that mediates the calcium sensing. The situation is however more complex, since the core channel contains binding sites for Ca2+ and Mg2+.
SEQUENCE SIMILARITIES: Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa1.1/KCNMA1 sub-subfamily. [View classification]
Contains 1 RCK N-terminal domain.
SEQUENCE CAUTION: The sequence AAA50215.1 differs from that shown. Reason: Erroneous initiation.产品使用声明 质量保证 Evaluated by Western Blot in rat brain membrane tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Slo1 on 10 µg of rat brain membrane tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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