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品种反应性 主要应用 宿主 格式 抗体类型 H WB M Purified Monoclonal Antibody 描述 产品目录编号 MABN59 Replaces MAB5657 描述 Anti-ACE2 Antibody, clone 4G5.1 Alternate Names - <br />Metalloprotease MPROT15
- Angiotensin-converting enzyme 2
- ACE-related carboxypeptidase
- Angiotensin-converting enzyme homolog
- ACEH
背景信息 ACE2 (Angiotensin-converting enzyme 2) is a transmembrane protein belonging to the peptidase M2 family. It is expressed in vascular endothelial cells of the heart, kidney, testis, and gastrointestinal system. ACE2 produces Angiotensin (1-9) by hydrolysis of Angiotensin 1. Angiotensin (1-9) is a precursor for Angiotensin (1-7). ACE2 may also directly produce Angiotensin (1-7) by conversion of Angiotensin II, which is involved in vasoconstriction. Angiotensin (1-7) plays an important role in the RAS signaling pathway and is involved in vasodilation. Hence, ACE2 antagonizes the effects of its homolog, ACE. ACE2 is insensitive to commonly used inhibitors such as linosipril and captopril. ACE2 is upregulated in heart failure, possibly in response to overactivity of Angiotensin II; ACE2 is studied for its potential therapeutic value for hypertension and heart failure. 产品信息 格式 Purified 控制 - HEK293 cell lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-ACE2 Antibody, clone 4G5.1 detects level of ACE2 & has been published & validated for use in Western Blotting. 主要应用 - Western Blotting
应用说明 ELISA Analysis: A representative lot detected ACE2 in a direct ELISA. 生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human ACE2. 克隆 4G5.1 宿主 Mouse 特异性 This antibody recognizes ACE2. 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_068576
Entrez基因汇总 The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. 基因符号 - ACE2
纯化方法 Protein G purified UniProt编号 - Q9BYF1
UniProt汇总 FUNCTION: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
CATALYTIC ACTIVITY: Angiotensin II + H2O = angiotensin-1-7 + L-phenylalanine.
COFACTOR: Binds 1 zinc ion per subunit. Binds 1 chloride ion per subunit.
ENZYME REGULATION: Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat.
SUBUNIT STRUCTURE: Interacts with ITGB1. Interacts with SARS-CoV and HCoV-NL63 spike glycoprotein.
SUBCELLULAR LOCATION: Processed angiotensin-converting enzyme 2: Secreted. Cell membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
INDUCTION: Up-regulated in failing heart.
POST-TRANSLATIONAL MODIFICATION: N-glycosylation on Asn-90 may limit SARS infectivity.
SEQUENCE SIMILARITIES: Belongs to the peptidase M2 family.产品使用声明 质量保证 Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected ACE2 in 10 µg of HEK293 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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