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品种反应性 主要应用 宿主 格式 抗体类型 H IHC, WB M Culture Supernatant Monoclonal Antibody 描述 产品目录编号 MABN527 描述 Anti-iNOS/NOS II, clone 13F5.1 Antibody Alternate Names - Peptidyl-cysteine S-nitrosylase NOS2
- Nitric oxide synthase, inducible
- Hepatocyte NOS
- HEP-NOS
- Inducible NO synthase
- Inducible NOS
- iNOS
- NOS type II
背景信息 iNOS/NOS II, also known as Nitric oxide synthase inducible, Hepatocyte NOS, HEP-NOS, Inducible NO synthase, Inducible NOS, iNOS, NOS type II, Peptidyl-cysteine S-nitrosylase , and encoded by the gene name NOS2 and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2). iNOS/NOS II is expressed in the liver, retina, bone cells and airway epithelial cells of the lung. 产品信息 格式 Culture Supernatant 演示 Mouse monoclonal IgG1λ in buffer containing 0.05% sodium azide. 应用 应用 This Anti-iNOS/NOS II antibody is validated for use in WB, IH for the detection of iNOS/NOS II. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected iNOS/NOS II in human cerebral cortex, human cerebellum, and human colon tissue. 生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human iNOS/NOS II. 克隆 13F5.1 浓缩 Please refer to lot specific datasheet. 宿主 Mouse 同种型 IgG1λ 品种反应性 Human Species Reactivity Note Human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_000616
基因符号 - NOS2A
- NOS2
纯化方法 Unpurified UniProt编号 - P35228
产品使用声明 质量保证 Evaluated by Western Blotting in human small intestine tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected iNOS/NOS II in 10 µg of human small Intestine tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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