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品种反应性 主要应用 宿主 格式 抗体类型 H, M, R ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABN125 描述 Anti-DRD4 Antibody, clone 2B9 Alternate Names - Dopamine D4 receptor
- D(4) dopamine receptor
- D(2C) dopamine receptor
背景信息 Five subtypes of dopamine receptors exist in two subfamilies: two D1-like (D1 and D5) and three D2-like (D2, D3 and D4). As members of the G protein-coupled receptor (GPCR) family, dopamine receptors initiate their biological function by coupling to GTP regulatory proteins (G proteins). D1-like receptors, DRD1 and DRD5, stimulate adenylyl cyclase whereas D2-like receptors, DRD2, DRD3 and DRD4, are coupled to inhibitory G proteins and their stimulation leads to the inhibition of adenylyl cyclase activity, and subsequent respective cellular signaling. All five subtypes have seven transmembrane domains, an extracellular amino terminus, and an intracellular carboxyl terminus. Allelic variants of the D4 dopamine receptor (DRD4) are known characterized risk factors of ADHD. It is reported that hypoxia enhances DRD4 promoter activity by several fold. It is also thought that dopamine receptors such as DRD4 are involved in neuroexcitation and in a variety of other neurological and psychiatric disorders. 产品信息 格式 Purified 控制 - Mouse brain tissue lysate.
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-DRD4 Antibody, clone 2B9 is validated for use in WB, IC for the detection of DRD4. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western Blot Analysis: 0.5 µg/mL from a representative lot detected DRD4 in 10µg of rat brain tissue lysate.
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected DRD4 in Ntera-2 cells and in rat brain neurons. (Wolstencroft, E.C., et al. (2007). J Mol Histol. 38(4):333-340.)生物信息 免疫原品种 Linear peptide corresponding to human DRD4. 克隆 2B9 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG1κ 品种反应性 HumanMouseRat Species Reactivity Note Demonstrated to react with Human, Mouse, and Rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_000788
Entrez基因汇总 This gene encodes the D4 subtype of the dopamine receptor. The D4 subtype is a G-protein coupled receptor which inhibits adenylyl cyclase. It is a target for drugs which treat schizophrenia and Parkinson disease. Mutations in this gene have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking. This gene contains a polymorphic number (2-10 copies) of tandem 48 nt repeats; the sequence shown contains four repeats. 基因符号 - DRD4
纯化方法 Protein G purified UniProt编号 - P21917
UniProt汇总 FUNCTION: This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
SUBUNIT STRUCTURE: Interacts with CLIC6 and GPRASP1.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.产品使用声明 质量保证 Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected DRD4 in 10µg of mouse brain tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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