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品种反应性 主要应用 宿主 格式 抗体类型 H FC, WB, IHC, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MABF29 描述 Anti-IL-16 Antibody, clone 14.1 Alternate Names - prointerleukin 16
- interleukin 16 (lymphocyte chemoattractant factor)
- lymphocyte chemoattractant factor
- pro-interleukin-16
- neuronal interleukin 16
背景信息 Originally known as lymphocyte chemoattractant factor (LCF), Interleukin-16 (IL-16) is a product of human peripheral mononuclear cells when antigen-stimulated or mitogen-stimulated. It is also produced by dendritic cells, epithelial cells, eosinophils, fibroblasts and mast cells. The pro-form of IL-16 is a 631 amino acid protein that is cleaved by caspase 3 in activated CD4+ T cells where it is then secreted and is accessible for binding CD4 receptors. IL-16 regulates and induces migratory response in CD4+ lymphocytes, eosinophils, and monocytes. It is a soluble ligand for CD4 and functions to prime responsiveness to IL-2 and IL-15 in CD4+ T-cells. Although Isoform 1’s function is thought to be as an anchor to membrane ion channels, Isoform 3 is more widely researched and characterized as component of T-cell cycle progression. 产品信息 格式 Purified 控制 - Human serum
演示 Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect IL-16 using this Anti-IL-16 Antibody, clone 14.1 validated for use in WB, NEUT, IH, IC, FC. 主要应用 - Flow Cytometry
- Western Blotting
- Immunohistochemistry
- Immunocytochemistry
应用说明 Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH to detect IL-16 in naive kidney tissue (Wang, S., et al., 2008).
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC to detect IL-16 in spinal cord cells (Skundric, D., et al., 2005).
Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC to detect IL-16 in human PBMCs (McFadden, C., et al., 2007).
Neutralizing Activity Assay: 1–10 µg/mL of a previous lot was used by an independent laboratory at concentrations sufficient to neutralize bioactivity of IL-16 (Morgan, R., et al., 2007).生物信息 免疫原品种 GST-tagged recombinant protein corresponding to the C-terminal region of secreted human IL-16. 表位 Unknown 克隆 14.1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001165599
Entrez基因汇总 The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq]. 基因符号 - prIL-16
- IL-16
- PRIL16
- LCF
- IL16
- NIL16
纯化方法 Protein G Purified UniProt编号 - Q14005
UniProt汇总 FUNCTION: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
SUBUNIT STRUCTURE: Homotetramer Probable; according (Ref.19) the formation of a homotetrameric protein complex is not required for the chemo-attractant function. Isoform 3 interacts (via PDZ 3 domain) with PPP1R12A, PPP1R12B and PPP1R12C. Isoform 1 interacts with PPP1R12B. Isoform 3 interacts with GRIN2A. Isoform 3 interacts with GABPB1. Isoform 3 interacts (via PDZ 3 domain) with HDAC3. Isoform 1 interacts with GRIN2D, KCNJ10, KCNJ15 and CACNA1C By similarity. Isoform 3 interacts with HTLV-1 tax.
SUBCELLULAR LOCATION: Interleukin-16: Secreted
Isoform 1: Cytoplasm (By similarity).
Isoform 3: Cytoplasm. Nucleus
TISSUE SPECIFICITY: Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T cell proliferation.
INDUCTION: Isoform 3 is down-regulated in T-cells after TCR activation.
PTM: Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16.
SEQUENCE SIMILARITIES: Contains 4 PDZ (DHR) domains.产品使用声明 质量保证 Evaluated by Western Blot in human serum.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected IL-16 in 10 µg of human serum.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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