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品种反应性 主要应用 宿主 格式 抗体类型 H FC, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 ABC505 描述 Anti-hERV-WL Antibody Alternate Names - SEC23-related protein B
- rWL-1
- Protein transport protein Sec23B
背景信息 Human endogenous retrovirus (HERV) proviruses comprise a significant part of the human genome, with approximately 98,000 ERV elements and fragments making up nearly 8% of the DNA. All appear to be inactive containing many deletions and nonsense mutations having integrated into the genome likely millions of years ago but left over HERV DNA as played an active role in genome evolution for millions and years and many important genes expressed today have promoters and enhances that have come from HERV DNA sequences. The best studied example is that of the retroviral fusogenic env proteins. In humans, and other mammals, intact env proteins called syncytins are responsible for the formation and function of syncytiotrophoblasts. These multi-nucleated cells are mainly responsible for maintaining nutrient exchange and protecting the developing fetus from the mother's immune system. The protein HERV-WL is an ancient retroviral envelope (env) protein. This endogenous envelope protein has retained its original fusogenic properties and participates in trophoblast fusion during placenta morphogenesis. HERV-WL gets cleaved into two functional chains upon expression. A surface protein named SU and a transmembrane protein called TM. SU mediates receptor recognition. This interaction triggers the refolding of the transmembrane protein (TM) and is thought to activate its fusogenic potential by unmasking its fusion peptide. The transmembrane protein (TM) acts as a class I viral fusion protein. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of membranes. HERV is expressed during development and in the uterine and placental tissues in the adult. 产品信息 格式 Serum 演示 Rabbit polyclonal serum in buffer containing 0.05% sodium azide. 应用 应用 Detect HERV using this rabbit polyclonal antibody, Anti-hERV-WL Antibody validated for use in western blotting & Flow Cytometry. 主要应用 - Flow Cytometry
- Western Blotting
应用说明 Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected hERV-WL in Jurkat cell lysate.
Flow Cytometry Analysis: 1 µL from a representative lot detected hERV-WL in 1X10E6 Raji and COS-7 cells.生物信息 免疫原品种 Recombinant protein corresponding to human ERV-WL. 宿主 Rabbit 品种反应性 Human 抗体类型 Polyclonal Antibody Entrez基因编号 - n/a
基因符号 - Sec23b
纯化方法 Unpurified UniProt编号 - P61561
产品使用声明 质量保证 Evaluated by Western Blotting in Tera-2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected hERV-WL in 10 µg of Tera-2 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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