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merck millipore,默克密理博,AB1786P,Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain
产品名称：Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain
产品型号：AB1786P
Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain detects level of Dopamine D3 Receptor & has been published & validated for use in ELISA, IH & WB.
merck millipore,默克密理博,AB1786P,Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, M WB, ELISA, IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB1786P 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain Alternate Names - D3R
产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA and 0.05% sodium azide. 应用 应用 Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain detects level of Dopamine D3 Receptor & has been published & validated for use in ELISA, IH & WB. 主要应用 - Western Blotting
- ELISA
- Immunohistochemistry
应用说明 Western blot: 1-10 μg/mL (Chemiluminescence technique; 45-50 kDa) Approximately 100-400 μg of membrane protein/lane should be loaded to visualize the receptor band. In some systems 70kDa bands may also be present.
Immunohistochemistry: suggested working concentration is 2-10 μg/mL. Suggested fixative is 4% PFA, fixation 10-15', permeabilized with 0.3-0.5% triton X-100 for 10-15' (tissue, less time for cels), primary antibody dilution is 1:100 for direct fluorescence . {Coronas, V, et al. J Neurochemistry 91:1292-1301, 2004},
ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Catalog Number AG229)/well)
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 A 19 amino acid peptide sequence from the rat D3 receptor within the 3rd cytoplasmic domain. 表位 cytoplasmic domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Specific for Dopamine Receptor D3. Rat Dopamine D3 receptor is a 446 aa, G-protein coupled and transmembrane receptor protein (Giros et al. 1990, 1991; Schmauss et al. 1993; Liu et al. 1994; Fu et al. 1995; Sokoloff et al. 1990; Livingstone et al. 1992). The immunogen peptide shows no significant homology with other dopamine receptors (D1, D2, D4 or D5).
SPECIES REACTIVITIES: The immunogen peptide shows sequence homology of 88% with mouse and 63% with human/monkey (12/19 aa) D3 receptors (Giros et al. 1990, 1991; Schmauss et al. 1993; Liu et al. 1994; Fu et al. 1995; Sokoloff et al. 1990; Livingstone et al. 1992). Antibody cross-reactivity with various species is not known.品种反应性 RatMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_033663.3
- NM_000796.3
Entrez基因汇总 This gene encodes the D3 subtype of the dopamine receptor. The D3 subtype inhibits adenylyl cyclase through inhibitory G-proteins. This receptor is expressed in phylogenetically older regions of the brain, suggesting that this receptor plays a role in cognitive and emotional functions. It is a target for drugs which treat schizophrenia, drug addiction, and Parkinson disease. Alternative splicing of this gene results in multiple transcript variants that would encode different isoforms, although some variants may be subject to nonsense-mediated decay (NMD). 基因符号 - D
- DRD3
- MGC149204
- D3DR
- ETM1
- MGC149205
- FET1
UniProt编号 - P35462
UniProt汇总 FUNCTION: SwissProt: P35462 # This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
SIZE: 400 amino acids; 44225 Da
SUBUNIT: Interacts with CLIC6 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Brain.
DISEASE: SwissProt: P35462 # Genetic variation in DRD3 may be associated with susceptibility to hereditary essential tremor 1 (ETM1) [MIM:190300]. ETM1 is the most common movement disorder. The main feature is postural tremor of the arms. Head, legs, trunk, voice, jaw, and facial muscles also may be involved. The condition can be aggravated by emotions, hunger, fatigue and temperature extremes, and may cause a functional disability or even incapacitation. Inheritance is autosomal dominant.
SIMILARITY: SwissProt: P35462 ## Belongs to the G-protein coupled receptor 1 family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
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