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品种反应性 主要应用 宿主 格式 抗体类型 H, M FC M Purified Monoclonal Antibody 描述 产品目录编号 MABF28 Replaces MABS174 描述 Anti-PAR-3 Antibody, clone 8E8 Alternate Names - partitioning-defective protein 3 homolog, Coagulation factor II receptor-like 2, Thrombin receptor-like 2
- Atypical PKC isotype-specific-interacting protein
- CTCL tumor antigen se2-5
- atypical PKC isotype-specific interacting protein
- par-3 (partitioning defective 3, C.elegans) homolog
- par-3 partitioning defective 3 homolog (C. elegans)
背景信息 PAR-3 (also known as Coagulation factor II receptor-like 2, Thrombin receptor-like 2 or F2RL2) is an adapter protein involved in asymmetrical cell division and cell polarization processes. It seems to play a central role in the formation of epithelial tight junctions. Its association with PARD6B may prevent the interaction of PAR-3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PAR-3 complex links GTP bound Rho small GTPases to atypical protein kinase C proteins. PAR-3 interacts with PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. PAR-3 interacts with PRCKI. PAR-3 forms part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. PAR-3 interacts with F11R/JAM1. Antibodies against PAR-3 are present in sera from patients with cutaneous T cell lymphomas. 产品信息 格式 Purified 控制 - Mouse platelets from washed whole blood (heparinized)
演示 Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-PAR-3 Antibody, clone 8E8 detects level of PAR-3 & has been published & validated for use in FC, EA. 主要应用 - Flow Cytometry
应用说明 Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC. (Petrova, Y., et al. (2008). Centr Eur J Immunol. 33 (1):14-18.)
Activity Assay Analysis (platelet aggregation): A previous lot was used by an independent laboratory in platelet aggregation assay. (Petrova, Y., et al. (2008). Centr Eur J Immunol. 33 (1):14-18.)生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human PAR-3. 克隆 8E8 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG2bκ 品种反应性 HumanMouse Species Reactivity Note Demonstrated to react with mouse.
Predicted to react with human based on 100% sequence homology.抗体类型 Monoclonal Antibody Entrez基因编号 - NP_004092
Entrez基因汇总 Coagulation factor II (thrombin) receptor-like 2 (F2RL2) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL2 is also a member of the protease-activated receptor family and activated by thrombin. F2RL2 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL2 is a cofactor for F2RL3 activation by thrombin. It mediates thrombin-triggered phosphoinositide hydrolysis and is expressed in a variety of tissues. [provided by RefSeq]. 基因符号 - F2RL2
- PAR3
- PARD3
- ASIP
- PHIP
- SE2-5T2
- PAR-3
- SE2-5L16
- PARD-3
- PARD3A
- PAR3A
- PAR3alpha
- PAR3-alpha
- Bazooka
- SE2-5LT1
纯化方法 Protein G Purified UniProt编号 - O00254
UniProt汇总 FUNCTION: Receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis.
SUBUNIT STRUCTURE: Interacts with INSC/inscuteable and probably GPSM2.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICTY: Highest expression in the megakaryocytes of the bone marrow, lower in mature megakaryocytes, in platelets and in a variety of other tissues such as heart and gut. PTM: A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
SEQUENCE SIMILARITIES: Belongs to the G-protein coupled receptor 1 family.
SEQUENCE CAUTION: The sequence CAD97628.1 differs from that shown. Reason: Frameshift at position 374.产品使用声明 质量保证 Evaluated by Flow Cytometry in mouse platelets from washed whole blood (heparinized).
Flow Cytometry Analysis: 2 µg of this antibody detected PAR-3 in mouse platelets from washed whole blood (heparinized).使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,05-1469,Anti-NFĸB Antibody, p65, clone 1G10.2
下一件merck millipore产品：merck millipore,默克密理博,MAB3412,Anti-Cytokeratin AE1/AE3 Antibody, recognizes acidic & basic cytokeratins, clone AE1/AE3
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