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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H IHC, FC M Purified Monoclonal Antibody 描述 产品目录编号 MABF250 Replaces 04-455 描述 Anti-CD20 Antibody, clone 2H7 Alternate Names - Membrane-spanning 4-domains subfamily A member 1
- B-lymphocyte antigen CD20
- B-lymphocyte surface antigen B1
- Bp35
- Leukocyte surface antigen Leu-16
背景信息 CD20 is a transmembrane, non-glycosylated protein expressed on B-cell precursors and mature B cells, but is lost following differentiation into plasma cells. In resting B cells, CD20 appears in a 33 kDa non-phosphorylated form. After mitogen stimulation, CD20 becomes heavily phosphorylated (35-37 kDa isoforms), and it is a dominant phosphoprotein in activated B cells and B-cell lines. The long N- and C-terminal ends of the protein are located on the cytoplasmic side of the membrane and only a minor portion of the protein is exposed on the cell surface. Antibodies reacting with CD20 cytoplasmic epitopes are designated CD20cy. It is suggested that CD20 plays a direct role in regulating the transmembrane conductive Ca2+ flux of B cells which indicates a possible function for CD20 as a regulator of proliferation and differentiation. 产品信息 格式 Purified 演示 Purified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect CD20 using this mouse monoclonal antibody, Anti-CD20 Antibody, clone 2H7 validated for use in Flow Cytometry, IP & IHC. 主要应用 - Immunohistochemistry
- Flow Cytometry
应用说明 Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated CD20 in Raji B cell lysate (Polyak, M. J., et al (2002). Blood. 99(9):3256-3262.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected CD20 in normal liver tissue, TPN-related cholestasis tissue and biliary artesia tissues (Mack, C. L., et al (2004). Pediatric Res. 56:79.).生物信息 免疫原品种 Recombinant protein corresponding to human CD20. 克隆 2H7 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG2bκ 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_068769
基因符号 - CD20
- MS4A1
纯化方法 Protein G purified UniProt编号 - P11836
产品使用声明 质量保证 Evaluated by Flow Cytometry in human PBMCs.
Flow Cytometry Analysis: 0.5 µg of this antibody detected CD20 in 1X10E6 human PBMCs.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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