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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Purified Monoclonal Antibody 描述 产品目录编号 04-772 Replaces 05-772 品牌系列 Upstate
商名 - Upstate
描述 Anti-Src Antibody, CT, clone NL19 | 04-772 Alternate Names - v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
- proto-oncogene tyrosine-protein kinase SRC
- protooncogene SRC, Rous sarcoma
- tyrosine kinase pp60c-src
- tyrosine-protein kinase SRC-1
- v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
背景信息 SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain. 产品信息 格式 Purified 控制 - RIPA whole cell lysates from human A431, Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells Included Positive Antigen Control: Catalog # 12-301, Non-Stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
演示 Cultured supernantant in 0.05% sodium azide 应用 主要应用 - Western Blotting
应用说明 Western Blot Analysis: A 1:2000 to 1:8000 dilution of this lot detected Src in RIPA whole cell lysates from human A431 cells. Immunoprecipitation: 5-20 μL of a previous lot immunoprecipitated Src from 500 μg of A431 RIPA lysate. 生物信息 免疫原品种 KLH conjugated synthetic peptide (CKVADFGLARLIEDNEYTARQGA) of Src (avian sarcoma virus), residues 400-422. The immunogen sequence shares 22/23 amino acids with Yes and Fyn, and 21/23 amino acids with Lyn and Hck. 克隆 NL19 宿主 Rabbit 特异性 Src-tyrosine kinase family 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Predicted to cross react with frog and chicken. The immunogen sequence shares 22/23 amino acids with Yes and Fyn, and 21/23 amino acids with Lyn and Hck. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_005417
Entrez基因汇总 This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. 基因符号 - pp60c-src
- SRC
- c-src
- p60-Src
- SRC1
- c-SRC
- ASV
UniProt编号 - P12931
UniProt汇总 SIZE: 536 amino acids; 59835 Da SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity. SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain. 产品使用声明 质量保证 routinely evaluated by immunoblot on RIPA whole cell lysates from human A431 and Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. 包装信息 数量 100 µL
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