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品种反应性 主要应用 宿主 格式 抗体类型 B, H, M, R, Rb WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 05-163 品牌系列 Upstate
商名 - Upstate
描述 Anti-PLCγ-1 Antibody 概述 An equal mix of IgG antibodies produced by SP2/0-Ag14 x Balb/c spleen cells1 and propagated as ascites. Clones B-2-5, B-6-4, B-20-3, D-7-3, E-9-4. Alternate Names - triphosphoinositide phosphodiesterase
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
- Phosphoinositide phospholipase C
- Phospholipase C-gamma-1
- monophosphatidylinositol phosphodiesterase
- phosphatidylinositol phospholipase C
- phosphoinositidase C
- phospholipase C gamma 1
- phospholipase C, gamma 1
- phospholipase C, gamma 1 (formerly subtype 148)
- phospholipase C-148
- phospholipase C-148
背景信息 Phospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown. 产品信息 格式 Purified 控制 - A431 cell lysate, HeLa cell lysate
Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
演示 Liquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide.
An equal mix of IgG antibodies produced by SP2/0-Ag14 x Balb/c spleen cells (Suh, P.G., 1988) and propagated as ascites. Clones B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.应用 应用 Anti-PLCγ-1 Antibody detects level of PLCγ-1 & has been published & validated for use in IC, IP & WB. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Immunoprecipitation:
4 µg of a previous lot of antibody immunoprecipitated PLCγ-1 from non-stimulated A431 cell lysate, previous lots immunoprecipitated PLCγ-1 from rabbit brain cytosol preparation.
Immunocytochemistry:
5-10 µg/mL of previous lots of this antibody detected PLCγ-1 on ethanol-acetic acid [95:5] fixed A431 cells.生物信息 免疫原品种 Five individual clones raised against purified soluble phospholipase C (PLC)γ-1 from bovine brain. 克隆 Clonal blend B-2-5, B-6-4, B-20-3, D-7-3, E-9-4. 宿主 Mouse 特异性 This antibody recognizes PLCγ-1, Mr 135 kDa. Reacts with some non-PLCγ-1 SH-3 containing proteins, including nck, Mr 47kDa (Park, D., 1992). 同种型 mixed IgGs 品种反应性 BovineHumanMouseRatRabbit Species Reactivity Note Bovine, rabbit, rat, mouse and human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002660.2
Entrez基因汇总 The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. 基因符号 - inositoltrisphosphohydrolase
- PLCG1
- PLC1
- PLCgamma1
- PLC148
- PLC-II
- PLC-148
- 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
- PLC-gamma-1
纯化方法 Protein G chromatography UniProt编号 - P19174
UniProt汇总 FUNCTION: SwissProt: P19174 # PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
COFACTOR: Calcium.
SIZE: 1290 amino acids; 148532 Da
SUBUNIT: Interacts with CENTG1 via its SH3 domain (By similarity). Interacts with phosphorylated LAT upon TCR activation. Interacts with the Pro-rich domain of TNK1 via its SH3 domain. Associates with BLNK, VAV1, GRB2 and NCK1 in a B-cell antigen receptor- dependent fashion. Interacts with CBLB in activated T-cells; which inhibits phosphorylation. Interacts with SHB. Interacts via its SH3 domain with the Arg/Gly-rich-flanked Pro-rich domains of KHDRBS1/SAM68. This interaction is selectively regulated by arginine methylation of KHDRBS1/SAM68. Interacts with INPP5D/SHIP1 (By similarity).
PTM: The receptor-mediated activation of PLC-gamma-1 and PLC- gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. & Ubiquitinated by CBLB in activated T-cells (By similarity).
SIMILARITY: SwissProt: P19174 ## Contains 1 C2 domain. & Contains 1 EF-hand domain. & Contains 2 PH domains. & Contains 1 PI-PLC X-box domain. & Contains 1 PI-PLC Y-box domain. & Contains 2 SH2 domains. & Contains 1 SH3 domain. ...产品使用声明 质量保证 Routinely evaluated in human A431 carcinoma cells, rabbit brain cytosol, and bovine brain cytosol preparations.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected PLCγ-1 in human A431 carcinoma cells; previous lots detected PKCγ-1 in rabbit brain cytosol and bovine brain cytosol preparations.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of shipment. 包装信息 数量 50 µg
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