- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
可用的分析物 品种反应性 主要应用 Ras R, H, M ELISA 描述 产品目录编号 17-497 Replaces 17-424 品牌系列 Upstate
商名 - Upstate
描述 Ras Activation ELISA ASSAY Kit 背景信息 Ras proteins function as GDP/GTP-regulated binary switches in signal transduction cascades that can lead to cell growth, proliferation, differentiation, or survival. This Ras superfamily has at least five major branches that include Ras, Rho, Ran, Arf/Sar, Rab. Ras itself has 3 primary isoforms (H-Ras, N-Ras, and K-Ras) that differ in their approximately 20 C-terminal amino acids. They are collectively referred to as Ras. In its active form, Ras is bound to GTP. This causes a conformational change that allow it to interact and bind to several effector molecules, most notably the members of the Raf family, the RalGDS family, and Phosphoinositide 3-kinases (PI3 Kinase). Ras then cleaves GTP to GDP resulting in its inactivation. In its oncogenic, mutated state, Ras is unable to hydrolyze GTP to GDP, thus staying in an active state and activating numerous pathways.
Due to its role as a key regulator of cellular functions and its implications in various cancers, Ras has been a popular target for cancer research and anti-cancer therapeutics for the past two decades for both academic and pharmaceutical research. It was also the first human oncogene identified. Mutations in cellular Ras have been found to be present in a large percentage of all human cancers. More specifically, K-Ras mutations occur frequently in lung, pancreatic, and colon cancers, where as H-Ras mutations are prevalent in bladder, kidney, and thyroid cancers, and N-Ras mutations are associated with melanoma, hepatocellular carcinoma, and leukemia (3). In recent studies, Ras activity has also been implicated in a number of human development defects such as Costello syndrome and Noonan syndrome (4).
One path that the pharmaceutical industry has taken to control Ras and its activity is by attacking what some consider its Achilles’ heel. For its activation, Ras must localize to the plasma membrane, but interestingly, it lacks a transmembrane domain. To achieve this, Ras must first undergo a post-translational modification known as prenylation or geranylation at its C-terminal CAAX motif. These drugs have yet to pass clinical trials though and there is doubt that they will ever be successful in treating tumors associated with Ras activation.
产品信息 部件 - 11. EGF stimulated HeLa Cell Lysate: (Part No. 12-500) 2 vials containing 40 µL of lysate at 5 mg/mL concentration, lysed in Mg2+ Lysis/Wash buffer (Catalog # CS202617).
- 1. Glutathione-Coated Plate, 96-well strip format: (Part No. 3007063) 1 plate containing 12 x 8 strip-wells coated with glutathione.
- 2. Anti-Ras Detection Antibody: (Part No. 2006992) One vial containing 10 μL of anti-Ras mouse monoclonal primary antibody. Can specifically detect K-, H-, N- isoforms of Ras.
- 3. Mg2+ Lysis/Wash Buffer, 5X: (Part No. CS202617) 1 vial containing 18 mL of 5X Mg2+ Lysis/Wash Buffer for preparation of cell lysate.
- 4. 20% Tween® 20: (Part No. # 20-246) One vial containing 3 mL of 20% Tween® 20.
- 5. 10% BSA in TBS (Blocking Buffer): (Part No. 20-191) One bottle containing 10 mL 10% BSA in 1X TBS.
- 6. 20X TBS Wash: (Part No. 20-190) One bottle containing 50 mL of 20X TBS, pH 7.4.
- 7. Gt x Ms, HRP: (Part No. CS202619) One amber vial containing 25 µL of goat anti-mouse HRP conjugated antibody.
- 8. Chemiluminescent Reaction Buffer: (Part No. 90496) One amber bottle containing 8 mL of Chemiluminescent Reaction Buffer. Keep out of light.
- 9. Chemiluminescent Detection Reagent: (Part No. 90495) One bottle vial containing 4 mL of Chemiluminescent Detection Reagent. Keep out of light.
- Store at -80º C
- 10. Raf-1-RBD, GST: (Part No. 14-863) 2 vials containing 150 μL of Raf-1-RBD at 2 μg/μL.
应用 主要应用 - ELISA
生物信息 品种反应性 RatHumanMouse 可用的分析物 - Ras
Entrez基因编号 - NP_002515.1
- NP_203524.1
- NP_001123914.1
Entrez基因汇总 K-Ras
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq]
H-Ras
This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq]基因符号 - neuroblastoma RAS viral (v-ras) oncogene homolog
- KRAS
- K-Ras
- v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
- K-ras p21 protein
- HRAS
- H-Ras
- Ha-Ras
- NRAS
- N-Ras
UniProt编号 - P01116
产品使用声明 质量保证 Routinely evaluated by ELISA. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
包装信息 数量 96 assays
上一件merck millipore产品：merck millipore,默克密理博,AB4130,Anti-FOXO1 Antibody
下一件merck millipore产品：merck millipore,默克密理博,14-504M,PKCδ Protein, active, 250 µg
相关产品
merck millipore,默克密理博,07-720,Anti-SNRK Antibody
merck millipore,默克密理博,444605,Melittin - CAS 37231-28-0 - Calbiochem
merck millipore,默克密理博,AB825,Anti-Apolipoprotein J Antibody
merck millipore,默克密理博,AB9553,Anti-Chemokine-Like Receptor 1 Antibody
merck millipore,默克密理博,AB4130,Anti-FOXO1 Antibody
merck millipore,默克密理博,14-504M,PKCδ Protein, active, 250 µg
merck millipore,默克密理博,MABF250,Anti-CD20 Antibody, clone 2H7
merck millipore,默克密理博,DR1050,Anti-NFE2L2 Mouse mAb (1F3)
merck millipore,默克密理博,04-772,Anti-Src Antibody, CT, clone NL19,04-772
merck millipore,默克密理博,EZBRAIN-SET,Human Amyloid β40 and Amyloid β42 Brain ELISA