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品种反应性 主要应用 宿主 格式 抗体类型 M, H IHC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABE78 Replaces MAB3747 描述 Anti-MiTF Antibody, clone 3D1.2 Alternate Names - bHLHe32
- Microphthalmia-associated transcription factor
- Class E basic helix-loop-helix protein 32
背景信息 Microphthalmia-associated transcription factor (MiTF) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, and development of mast cells and bone. Mutations in MiTF cause auditory pigmentary syndromes in humans, such as Waardenburg syndrome type II and type IIa, and Tietz syndrome. There are two known isoforms of MiTF, differing by 66 amino acids at the N-terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, whereas the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. 产品信息 格式 Purified 控制 - Mouse brain tissue lysate
演示 Purified mouse monoclonal IgMκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-MiTF Antibody, clone 3D1.2 is an antibody against MiTF for use in Western Blotting & IHC. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected MiTF in human melanoma tissue. 生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human MiTF. 克隆 3D1.2 宿主 Mouse 同种型 IgMκ 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with Human and Mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_000239
基因符号 - BHLHE32
- MITF
纯化方法 Purified by ion-exchange chromatography UniProt编号 - O75030
产品使用声明 质量保证 Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected MiTF in 10 µg of mouse brain tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 200 µL
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