merck millipore,默克密理博,MABE548,Anti-Cdk4, clone EPR4513, Rabbit Monoclonal Antibody

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merck millipore,默克密理博,MABE548,Anti-Cdk4, clone EPR4513, Rabbit Monoclonal Antibody
产品名称：Anti-Cdk4, clone EPR4513, Rabbit Monoclonal Antibody
产品型号：MABE548
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,MABE548,Anti-Cdk4, clone EPR4513, Rabbit Monoclonal Antibody

产品介绍

merck millipore,默克密理博,MABE548,Anti-Cdk4, clone EPR4513, Rabbit Monoclonal Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	IHC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	MABE548
描述	Anti-Cdk4, clone EPR4513, Rabbit Monoclonal Antibody
Alternate Names	Cyclin-dependent kinase 4
背景信息	Cyclin-dependent kinase 4, aslo known as Cell division protein kinase 4 or PSK-J3, and encoded by the gene name CDK4, is a member of the CMGC Ser/Thr protein kinase family. Cyclin dependent kinases (CDK's) are kinases that interact with cyclins and regulate cell division. There is evidence that phosphorylation of the C-terminal region of Rb by Cdk4/6 initiates successive intramolecular interactions between the C-terminal region and the central pocket. The initial interaction displaces histone deacetylase from the pocket, blocking active transcriptional repression by Rb. These intramolecular interactions provide a molecular basis for sequential phosphorylation of Rb by Cdk4/6 and Cdk2. Cdk4/6 is activated early in G1, blocking active repression by Rb. In primary epidermal cells, it has been found that oncogenic Ras transiently decreases cyclin-dependent kinase (CDK) 4 expression in association with cell cycle arrest in G1 phase. CDK4 co-expression circumvents Ras growth suppression and induces invasive human neoplasia resembling squamous cell carcinoma. Tumorigenesis is dependent on CDK4 kinase function, with cyclin D1 required but not sufficient for this process. These data identify a new role for oncogenic Ras in CDK4 regulation and highlight the functional importance of CDK4 suppression in preventing uncontrolled growth.

产品信息
格式	Unpurified
演示	Rabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Immunohistochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:100-250 dilution from a representative lot detected Cdk4 in human urothelial carcinoma. Produced in collaboration with Epitomics™

生物信息
免疫原品种	Synthetic peptide corresponding to the C-terminus of human Cdk4.
表位	C-terminus
克隆	EPR4513
浓缩	Please refer to lot specific datasheet.
宿主	Rabbit
同种型	IgG
品种反应性	Human
Species Reactivity Note	Human
抗体类型	Monoclonal Antibody
基因符号	CDK4
纯化方法	Unpurified
UniProt编号	P11802

产品使用声明
质量保证	Evaluated by Western Blotting in HeLa, MCF-7, K562, and Ramos cell lysates. Western Blotting Analysis: A 1:1,000-10,000 dilution of this antibody detected Cdk4 in HeLa, MCF-7, K562, and Ramos cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.