merck millipore,默克密理愽
  • merck millipore,默克密理博,AB1560,Anti-Opioid Receptor Antibody, δ, pain, NT

  • merck millipore,默克密理博,AB1560,Anti-Opioid Receptor Antibody, δ, pain, NT

    产品名称：Anti-Opioid Receptor Antibody, δ, pain, NT
    产品型号：AB1560
    Anti-Opioid Receptor Antibody, δ, pain, N-terminus detects level of Opioid Receptor & has been published & validated for use in FC, IH(P) & WB.

    merck millipore,默克密理博,AB1560,Anti-Opioid Receptor Antibody, δ, pain, NT

  • 产品介绍
  • merck millipore,默克密理博,AB1560,Anti-Opioid Receptor Antibody, δ, pain, NT

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    H, M, RWB, FCRbSerumPolyclonal Antibody
    描述
    产品目录编号 AB1560
    Replaces MAB5330
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Opioid Receptor Antibody, δ, pain, NT
    产品信息
    格式 Serum
    控制
    • Spinal cord
    演示 UnPurified whole rabbit antiSerum containing no preservatives.
    应用
    应用 Anti-Opioid Receptor Antibody, δ, pain, N-terminus detects level of Opioid Receptor & has been published & validated for use in FC, IH(P) & WB.
    主要应用
    • Western Blotting
    • Flow Cytometry
    应用说明 Immunohistochemistry on CNS tissues fixed in PLP: 1:1,000.

    Reactive on paraffin embedded tissue.

    Western Blot: 1:1000. Under reducing conditions the antibody will detect major bands at 36 and 72 kDa.

    FACS on human T-cells (Sharp, 2001)

    Optimal working dilutions must be determined by the end user.

    IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1560

    This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

    1) 3 x 10 minute washes in TBS (without Triton).

    2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

    3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (without Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

    4) 3 x 10 minute washes in TBS.

    5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody).

    6) 3 x 10 minute washes in TBS.

    7) ABC Elite (1:200 Vector Labs) in TBS.

    8) 2 x 10 minute washes in TBS.

    9) 1 x 10 minute wash in phosphate buffer (no saline).

    10) DAB reaction with 0.06% NiCl added for intensification.

    11) 2 x 10 minute washes in PBS.

    12) 1 x 10 minute wash in phosphate buffer (no saline).
    生物信息
    免疫原品种 Delta opioid receptor N-terminal peptide (LVPSARAELQSSPLV).
    表位 N-terminus
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Recognizes Delta opioid receptor. The control peptide (catalog number AG288) is available for use in antibody blocking studies.
    品种反应性 HumanMouseRat
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_000911.3
    基因符号
    • DOR-1
    • OPRD1
    • OPRD
    纯化方法 Unpurified
    UniProt编号
    • P41143
    UniProt汇总 FUNCTION: SwissProt: P41143 # Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Highly stereoselective. receptor for enkephalins.
    SIZE: 372 amino acids; 40413 Da
    SUBUNIT: Interacts with GPRASP1.
    SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
    SIMILARITY: SwissProt: P41143 ## Belongs to the G-protein coupled receptor 1 family.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
    包装信息
    数量 50 µL

    merck millipore,默克密理博,AB1560,Anti-Opioid Receptor Antibody, δ, pain, NT

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