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品种反应性 主要应用 宿主 格式 抗体类型 H IHC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABE362 Replaces 04-1047 描述 Anti-EZH2 Antibody, clone AE25 Alternate Names - histone-lysine N-methyltransferase EZH2
- Enhancer of zeste homolog 2
- Lysine N-methyltransferase 6
- enhancer of zeste 2
背景信息 EZH2 (enhancer of zeste homologue 2), also known as Enx1, is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. EZH2 is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, EZH2 is restricted to the spleen, testis, and placenta. EZH2 and BMI-1 genes are coexpressed in Reed-Sternberg cells of Hodgkin’s disease. Coexpression of BMI-1 and EZH2 is also associated with cycling cells and degree of malignancy in B-cell non-Hogkin’s lymphoma. EZH2 is involved in the progression of prostate cancer, and has been identified as a marker of aggressive breast cancer. Two transcript variants encoding distinct isoforms have been identified for this gene. 产品信息 格式 Purified 控制 - T47D cell lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-EZH2 Antibody, clone AE25 is validated for use in Western Blotting, IHC for the detection of EZH2. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Western Blot Analysis: A 1:500 dilution from a representative lot detected EZH2 in Jurkat and HeLa cell lysates.
Immunohistochemistry Analysis: A representative lot detected EZH2 in breast carcinoma tissue (Pietersen et al., 2008)生物信息 免疫原品种 MBP-tagged recombinant protein corresponding to human EZH2. 克隆 AE25 宿主 Mouse 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001190176
基因符号 - KMT6
- ENX-1
纯化方法 Protein G purified UniProt编号 - Q15910
产品使用声明 质量保证 Evaluated by Western Blot in T47D cell lysate.
Western Blot Analysis: A 1:1000 dilution of this antibody detected EZH2 in 10 µg of T47D cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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