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品种反应性 主要应用 宿主 格式 抗体类型 M, Ma, H, R WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1492 Replaces AB1696 描述 Anti-Calcineurin Aα Antibody Alternate Names - nuclear factor of activated T-cells, cytosolic component 1
- NFAT transcription complex cytosolic component
- nuclear factor of activated T-cells, cytoplasmic,
- calcineurin-dependent 1
背景信息 Calcineurin is a serine/threonine protein phosphatase involved in the mediation of signal transduction in a wide variety of cellular responses. Calcineurin has been linked to Pmk1 MAP kinase and PI pathways, as well as to members of the small GTPase Rab/Ypt family and Type II myosin. Furthermore, calcineurin participates in the transduction of extracellular signals to the nucleus by dephosphorylation of members of the NFAT family of transcription factors. These linkages demonstrate calcineurin's multifunctional capabilities in the regulation of calcium signaling. The Aα form of calcineurin is a major catalytic subunit gene isoform of calcineurin in most tissues that allows differentiation of Aα from the Aβ and Aγ isoforms. 产品信息 格式 Affinity Purified 控制 - Mouse brain lysate
演示 Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 40% gylerol. Contains no preservative. 应用 应用 This Anti-Calcineurin Aα Antibody is validated for use in WB for the detection of Calcineurin Aα. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugagted synthetic peptide corresponding to Calcineurin Aα. 宿主 Rabbit 特异性 This antibody recognizes the Aα form of calcineurin. 品种反应性 MouseMammalsHumanRat Species Reactivity Note Reacts with human, mouse, and rat. Predicted to react with all mammals based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_000935.1
基因符号 - NFATc
- MGC138448
- NF-ATC
- NF-ATc
- NF-ATc1
- NFAT2
- NFATC
纯化方法 Affinity Purfied UniProt编号 - Q08209
UniProt汇总 FUNCTION: Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.
SUBUNIT STRUCTURE: Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
TISSUE SPECIFICITY: Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.
INDUCTION: Only isoforms A are inducibly expressed in T lymphocytes upon activation of the T-cell receptor (TCR) complex. Induced after co-addition of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Also induced after co-addition of 12-O-tetradecanoylphorbol-13-acetate (TPA) and ionomycin. Weakly induced with PMA, ionomycin and cyclosporin A.
DOMAIN: Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
The N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus.
Isoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor.
PTM: Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
SEQUENCE SIMILARITIES: Contains 1 RHD (Rel-like) domain.产品使用声明 质量保证 Evaluated by Western Blot in Mouse brain lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Calcineurin on 10 µg of mouse brain lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at -20°C for up to 12 months in undiluted aliquots; at this temperature the solution will
remain unfrozen.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.包装信息 数量 100 µL
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