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merck millipore,默克密理博,MABE282,Anti-c-Myc Antibody, clone 9E10
产品名称：Anti-c-Myc Antibody, clone 9E10
产品型号：MABE282
Anti-c-Myc Antibody, clone 9E10 is a Mouse Monoclonal Antibody for detection of c-Myc also known as Myc proto-oncogene protein, HLHe39, Transcription factor p64 & has been validated in WB, ChIP.
merck millipore,默克密理博,MABE282,Anti-c-Myc Antibody, clone 9E10
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ChIP, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABE282 描述 Anti-c-Myc Antibody, clone 9E10 Alternate Names - Transcription factor p64
- Myc proto-oncogene protein
- Class E basic helix-loop-helix protein 39
- HLHe39
- Proto-oncogene c-Myc
背景信息 c-Myc (bHLHe39) is a transcription factor that regulates the expression of multiple genes involved in apoptosis, cell differentiation and cell proliferation. Active c-Myc is coupled to the MAX protein via c-Myc’s C-terminal helix-loop-helix leucine zipper (bHLHLZ) domain. The c-Myc-MAX heterodimer also interacts with a number of other transcription-related proteins including TRRAP, p107 and Miz-1, and c-Myc-MAX may indirectly regulate histone acetylases involved in chromatin remodeling to increase accessibility of transcriptional complexes to target DNA sequences. The expression of c-Myc is controlled by growth factors. Several studies have reported abnormal expression of c-Myc in various cancers including Burkitt’s lymphoma. 产品信息 格式 Purified 控制 - HeLa cell lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-c-Myc Antibody, clone 9E10 is a Mouse Monoclonal Antibody for detection of c-Myc also known as Myc proto-oncogene protein, HLHe39, Transcription factor p64 & has been validated in WB, ChIP. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
应用说明 Western Blot Analysis: A representative lot from an independent laboratory detected c-Myc in various cell lysates (Evan, G. I., et al. (1985). Mol Cell Biol. 5(12):3610-3616).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected c-Myc in ChIP (Sanyal, K., et al. (2004). Proc Natl Acad Sci USA. 101(31):11374-11379).生物信息 免疫原品种 Linear peptide corresponding to human c-Myc. 克隆 9E10 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_002458
Entrez基因汇总 The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. 基因符号 - BHLHE39
- MYC
纯化方法 Protein G purified UniProt编号 - P01106
UniProt汇总 FUNCTION: Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
SUBUNIT STRUCTURE: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with KDM5A and KDM5B. Interacts (when phosphorylated at Thr-58 and Ser-62) with FBXW7. Interacts with PIM2.
SUBCELLULAR LOCATION: Nucleus > nucleoplasm. Nucleus > nucleolus.
POST-TRANSLATIONAL MODIFICATION: Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC. Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence.
Ubquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
INVOLVEMENT IN DISEASE: Note: Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. Note: A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1. Defects in MYC are a cause of Burkitt lymphoma (BL). A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
BIOTECHNOLOGY USE: POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differenciated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
SEQUENCE SIMILARITIES: Contains 1 basic helix-loop-helix (bHLH) domain.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected c-Myc in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 200 µg
上一件merck millipore产品：merck millipore,默克密理博,05-843R,Anti-phospho-Bcl-2 (Ser70) Antibody, clone 69-10C-2-10C-18, rabbit monoclonal
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