merck millipore,默克密理博,MABE229,Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
H	ICC, WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	MABE229
描述	Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2
Alternate Names	p34 protein kinase Cyclin-dependent kinase 1 CDK1 Cell division control protein 2 homolog Cell division protein kinase 1
背景信息	Cyclin dependent kinase 1 (Cdk1) regulates cell cycle progression. It is active as a heterodimeric complex with cyclins. Cdk1 associates with cyclin A at the end of S phase and targets proteins such as p53, BARD1, and BRCA2 involved in cell cycle progression, and others involved in DNA replication. At the end of the G2 phase, Cdk1 associates with cyclin B1 and B2 to phosphorylate multiple protein targets required for transition from G2 to M phase, and progression through M phase. Cdk1 itself undergoes phosphorylation at Thr14 and Tyr15 by PKMYT1, which prevents nuclear translocation of Cdk1. Cdk1 is also phosphorylated at Tyr15 by WEE1/2 which inhibits both Cdk1 activity and transition from G2 to M phase. Conversely, dephosphorylation of Thr14 and Tyr15 by CDC25A/B activates Cdk1 and triggers mitosis. Cdk1 may also regulate epigenetic gene silencing through phosphorylation of EZH2 and maintenance of histone H3 lysine 27 trimethylation, which has been implicated in cancer.

产品信息
格式	Purified
控制	UV treated and untreated HeLa cell lysates
演示	Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	Anti-phospho-Cdk1 (Thr14, Tyr15) Antibody, clone CP3.2 is a mouse monoclonal antibody for detection of phospho-Cdk1 (Thr14, Tyr15) also known as Cyclin-dependent kinase 1, or Cell division control protein 2 homolog & has been validated in WB, ICC.
主要应用	Immunocytochemistry Western Blotting

生物信息
免疫原品种	Linear peptide corresponding to human Cdk1 phosphorylated at Thr14 and phosphorylated at Tyr15.
表位	Phosphorylated Thr14 and phosphorylated Tyr15
克隆	CP3.2
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	This antibody recognizes Cdk1 phosphorylated at Thr14 and phosphorylated at Tyr15.
同种型	IgG1κ
品种反应性	Human
Species Reactivity Note	Demonstrated to react with Human.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_001777
基因符号	P34CDC2 CDK1 CDC2 CDC28A CDKN1
纯化方法	Protein G purified
UniProt编号	P06493

产品使用声明
质量保证	Evaluated by Western Blot in UV treated and untreated HeLa cell lysates. Western Blot Analysis: A 1:1,000 dilution from a representative lot detected phospho-Cdk1 (Thr14, Tyr15) in UV treated HeLa cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt.

包装信息
数量	100 µL

merck millipore,默克密理博,MABE229,Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2

whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech