merck millipore,默克密理博,MABE214,Anti-NEDD8 (NT) Antibody, clone Y297, rabbit monoclonal

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merck millipore,默克密理博,MABE214,Anti-NEDD8 (NT) Antibody, clone Y297, rabbit monoclonal
产品名称：Anti-NEDD8 (NT) Antibody, clone Y297, rabbit monoclonal
产品型号：MABE214
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,MABE214,Anti-NEDD8 (NT) Antibody, clone Y297, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABE214,Anti-NEDD8 (NT) Antibody, clone Y297, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	IHC, WB, ICC	Rb	Diluted Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	MABE214
描述	Anti-NEDD8 (NT) Antibody, clone Y297, rabbit monoclonal
Alternate Names	Neural precursor cell expressed developmentally down-regulated protein 8 neural precursor cell expressed, developmentally down-regulated 8 Ubiquitin-like protein Nedd8
背景信息	NEDD8 (Neural precursor cell-Expressed Developmentally Down-regulated genes) is an ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis (1). NEDD8 has been shown to conjugate to nuclear proteins in a manner analogous to ubiquination and sentrinization. The conjugation process is though to be catalyzed by thee enzymes; E1 (NEDD8-activation), E2 (NEDD8-conjugation) and E3 (NEDD8-ligation). NEDD8 is known to conjugate to Cul-4A and other cullins proteins via the carboxy-terminal Gly residue for ubiquitinylation (3) while NUB1 will negatively regulate its expression (4).

产品信息
格式	Diluted Culture Supernatant
控制	HeLa and K562 cell lysates
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Immunohistochemistry Western Blotting Immunocytochemistry
应用说明	Immunocytochemistry Analysis: 1:100-1:250 dilution from a representative lot was used in IC. Immunoprecipitation Analysis: 1:50 dilution from a representative lot was used in IP. Immunohistochemistry Analysis: 1:100-1:250 dilution from a representative lot detected NEDD8 in human lung tissue.

生物信息
免疫原品种	Synthetic peptide corresponding to the N-terminus of human NEDD8.
克隆	EP1513Y
宿主	Rabbit
特异性	This antibody recognizes NEDD8 at the N-terminus. It also detects NEDD8 bound to cullins proteins (neddylated cullins) as well as free NEDD8.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Demonstrated to react with human.
抗体类型	Monoclonal Antibody
基因符号	Neddylin NEDD-8 Nedd-8 MGC125897 neddylin MGC125896 FLJ43224 MGC104393
纯化方法	Unpurified
UniProt编号	Q15843
UniProt汇总	FUNCTION: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. SUBUNIT STRUCTURE: Directly interacts with NUB1 and AHR. Covalently attached to cullins and p53. SUBCELLULAR LOCATION: Nucleus. Note: Mainly nuclear. TISSUE SPECIFICITY: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes. PTM: Cleavage of precursor form by UCHL3 or SENP8 is necessary for function. SEQUENCE SIMILARITIES: Belongs to the ubiquitin family.

产品使用声明
质量保证	Evaluated by Western Blot in HeLa and K562 cell lysates. Western Blot Analysis: 1:500 dilution of this antibody detected NEDD8 in HeLa and K562 cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing Solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below
-20°C may cause glycerol containing Solutions to become frozen during storage.