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merck millipore,默克密理博,17-641,ChIPAb+ REST - ChIP Validated Antibody and Primer Set
产品名称：ChIPAb+ REST - ChIP Validated Antibody and Primer Set
产品型号：17-641
REST ChIP validated antibody & primer set including the ChIP-grade antibody & the specific control PCR primers used for chromatin immunoprecipitation of REST.
merck millipore,默克密理博,17-641,ChIPAb+ REST - ChIP Validated Antibody and Primer Set
- 产品介绍
重要规格表
品种反应性 主要应用 R, H, M ChIP, WB 描述 产品目录编号 17-641 品牌系列 Upstate
商名 - Upstate
- ChIPAb+
描述 ChIPAb+ REST - ChIP Validated Antibody and Primer Set 概述 All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context.
Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ REST set includes the REST antibody, a negative control antibody (purified rabbit IgG), and positive qPCR primers which amplify a 62 bp region near the RE1 site of the human synapsin gene. The REST and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of REST-associated chromatin.Alternate Names - repressor binding to the X2 box
- Neural-restrictive silencer factor
- RE1-silencing transcription factor
- X2 box repressor
- neuron restrictive silencer factor
背景信息 Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression. 产品信息 格式 Affinity Purified 控制 - Includes negative control rabbit IgG antibody and control primers specific for the RE1 site of human Synapsin.
演示 Anti-REST (rabbit polyclonal IgG). One vial containing 50 μg of affinity purified antibody in 50 μL PBS with 0.07% sodium azide, and 30% glycerol. Store at -20°C.
Normal Rabbit IgG. One vial containing 125 μg purified rabbit IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
ChIP Primers, Synapsin RE1. One vial containing 75 μL of 5 μM of each primer specific for the RE1 site of human Synapsin. Store at -20°C.
FOR: GGT GCT GAA GCT GGC AGT
REV: TGG GTT TTA GGA CCA GGA TG
应用 应用 REST ChIP validated antibody & primer set including the ChIP-grade antibody & the specific control PCR primers used for chromatin immunoprecipitation of REST. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
应用说明 Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa S3 cells (4 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 2 μg of either a normal rabbit IgG or Anti-REST antibody and the EZ-Magna ChIP A Kit (Part No. 17-408). Successful immunoprecipitation of REST-associated DNA fragments was verified by qPCR using primers for CDC25 as a negative control and positive control Primers Syn RE1 (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna A ChIP™ (Cat. # 17-408) or EZ-ChIP ™ (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
HeLa nuclear extract was resolved by electrophoresis, transferred to PVDF membrane and probed with the Ab at a dilution of 1:1000.生物信息 免疫原品种 The REST purified antibody is made against a GST fusion protein corresponding to amino acids 801-1097 of human REST. 表位 a.a. 801-1097 宿主 Rabbit 特异性 Recognizes REST, Mr 120-200 kDa. 品种反应性 RatHumanMouse Species Reactivity Note Human, mouse, rat. Not tested in other species. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_005603
Entrez基因汇总 This gene encodes a transcriptional repressor which represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells, and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described; however, their full length nature has not been determined. [provided by RefSeq].
基因符号 - XBR
- NRSF
UniProt编号 - Q13127
UniProt汇总 FUNCTION:Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
SUBUNIT STRUCTURE: Interacts with SIN3A, SIN3B and RCOR1.
SUBCELLULAR LOCATION: NucleusProbable.
TISSUE SPECIFICITY: Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
SEQUENCE SIMILARITY:Contains 9 C2H2-type zinc fingers.
SEQUENCE CAUTION: The sequence AAA98503.1 differs from that shown. Reason: Frameshift at positions 189, 212 and 1087.
The sequence AAH38985.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.
产品使用声明 质量保证 Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa S3 cells (4 x 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal rabbit IgG or Anti-REST antibody and the Magna ChIP A Kit (Part No. 17-610) (Please see figures). Successful immunoprecipitation of REST-associated DNA fragments was verified by qPCR using Control Primers.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. 包装信息 数量 25 assays 包装 25 assays per set. Recommended use: ~2 μg antibody per chromatin immunoprecipitation (dependent upon biological context).
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